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title page
Contents
Introduction 9
Objective 11
Materials and Methods 12
Animals 12
Radiofrequency Procedure 13
Study Groups 14
Phase 1: 0.5 ㎝ (group A) vs. 1.0 ㎝ (group B) safe distance 16
Phase 2: vertical vs. parallel direction of electrode to the GB wall 16
Phase 3: immediate vs. acute phase 17
Analysis of GB wall injury 19
I. Gross Pathologic Analysis 19
II. Histopathologic Analysis 19
Statistical Analysis 21
Results 22
I. Gross Changes of the GB Wall 22
Phase 1: 0.5 ㎝ (group A) vs. 1.0 ㎝ (group B) safe distance 22
Phase 2: vertical vs. parallel direction of electrode to the GB wall 26
Phase 3: immediate vs. acute phase 26
II. Microscopic Changes of the GB Wall 27
A. Depth of Thermal Injury of the GB Wall 27
Phase 1: 0.5 ㎝ (group A) vs. 1.0 ㎝ (group B) safe distance 27
Phase 2: vertical or parallel direction of electrode to the GB wall 31
Phase 3: immediate vs. acute phase 31
B. Variables of Abnormal Microscopic Changes of GB Mucosa 33
Discussion 36
Conclusion 42
References 43
English Abstracts 50
Korean Abstracts 53
Table 1. The Number of RF Ablation Zones Created in 15 Pigs Liver according to Three Phases 15
Table 2. Gross Changes of Gallbladder Wall Adjacent to Pig Liver after RF Ablation without Safe Distance (Group A) and with Safe Distance (Group B) Based on Electrode Direction and Time of Sacrifice. 23
Table 3. The Depth of Thermal Injury of Abutting Gallbladder Wall adjacent to Ablation Zones in Pig Liver without Safe Distance (Group A) and with Safe Distance (Group B) Based on Electrode Direction and Time of Sacrifice. 32
Table 4. The Grade of Microscopic Changes found in Gallbladder Mucosa adjacent to Ablation Zones Created in Pig Livers after RF Ablation without Safe Distance (Group A) and with Safe Distance (Group B) 34
Figure 1. The method of electrode direction to the GB in a pig liver. 18
Figure 2. Photographs of the specimens extracted from the group A pig immediately after RF ablation with parallel direction (group Ai and Ap). 24
Figure 3. Photographs of the specimens extracted from group A pig seven days following RF ablation with parallel direction (group Aa and Ap).... 25
Figure 4. Photomicrographs of resected GB immediately following RF ablation with parallel direction in no safe distance group (group Ai and Ap).... 28
Figure 5. Photomicrographs of resected GB seven days following RF ablation with parallel direction in safe distance group (group Ba and Bp).... 29
Figure 6. Photomicrographs of resected GB immediately following RF ablation with parallel direction in no safe distance group (group Ai and Ap).... 30
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