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22대 대한민국 국회 국회정보길라잡이 국회의원검색
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정보검색

소장정보 검색

국회도서관 홈으로 정보검색 소장정보 검색
결과 내 검색 동의어 포함
결과 내 검색 동의어 포함

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title page

Contents

List of abbreviations 10

General Introduction : Characterization of fibroin gene in silkworm 11

1. Silkworm 12

2. Fibroin gene and promoter 14

3. Transcriptional regulation of fibroin gene 15

4. Transcription factors of posterior silk gland in Bombyx mori 16

5. Transcriptional regulation 17

5.1. Activation of chromosome structure 17

5.2. Regulation in the transcription level 18

5.2.1. Transcriptional activation (enhancer) 19

5.2.2. Transcriptional repression 20

6. Expression of fibroin gene 22

7. Transgenic silkworms 23

8. Transposon-mediated gene transfer 25

8.1. P element 25

8.2. piggyBac 26

9. The expression of the transgenes 27

10. Analysis for protein binding sites in promoter 28

Chapter 1. Structural characterization of putative transcription factor of silkworm, Bombyx mori 29

Introduction 30

Materials and methods 33

Results 40

Discussion 55

Chapter 2. Construction of transgenic silkworm producing epidermal growth factor in Bombyx mori 59

Introduction 60

Materials and Methods 63

Results 70

Discussion 87

References 89

ABSTRACT 118

국문요약 121

List of Tables

General Introduction 9

Chapter 1 9

Table 1. Expression of silk proteins in posterior silk gland using ESTs data 44

Table 2. Putative transcription factors in posterior silk gland using ESTs data 45

Table 3. Primer sequences for amplification of the using probe 47

Chapter 2 9

Table 1. Injection and transformation of p[FPEGF] vector and study of EGF transgenic animals 74

Table 2. The comparison with normal pupae and transgenic pupae weight 78

List of Figures

General Introduction 7

Chapter 1 7

Fig. 1. cDNA sequence of EST data used as probes. 8-10, 8-83 and peg053 are names in EST data of B. mori. The three ESTs were partially matched with transcription factor of other organisms (shown in round brackets). 46

Fig. 2. Construction of genomic library and plaque hybridization. 48

Fig. 3. Confirmation of probes by PCR with the genomic DNA. 49

Fig. 4. RT-PCR of silkworm according to specific tissues. 50

Fig. 5. Hypothetical structure of counterpart of p8 in B. mori. 51

Fig. 6. Hypothetical structure of counterpart of ASF in B. mori. 52

Fig. 7. Comparisons of hypothetical Asf1 of B. mori. 53

Fig. 8. Comparisons of hypothetical p8 of B. mori. 54

Chapter 2 8

Fig. 1. Construction of expression vector p[FPEGF]. 71

Fig. 2. Analysis of genomic PCR of transgenic silkworm. 75

Fig. 3. Results of comparison with PCR products sequence of transgenic silkworms F2 and EGF gene/SV40 poly (A) signal with used for foreign DNA in microinjection.... 76

Fig. 4. The comparison with normal pupae size and transgenic pupae size. 77

Fig. 5. Result of inverse PCR of genomic DNA in transgenic silkworm. After inverse PCR, we sequenced. The sequence was subjected to BLAST analysis against the nucleotide sequence data present in GenBank. 80

Fig. 6. RT-PCR of transgenic silkworm according to specific tissues. 82

Fig. 7. Dot blot analysis. 84

Fig. 8. ELISA analysis 86

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