생몰정보
소속
직위
직업
활동분야
주기
서지
국회도서관 서비스 이용에 대한 안내를 해드립니다.
검색결과 (전체 1건)
원문 있는 자료 (1) 열기
원문 아이콘이 없는 경우 국회도서관 방문 시 책자로 이용 가능
목차보기더보기
표제지
목차
ABSTRACT 10
Abbreviation 12
I. INTRODUCTION 14
II. MATERIALS AND METHODS 19
1. Subjects 19
2. Blood collection and preparation for peripheral blood cells culture 20
3. RAST (Radioallergoaorbent test) inhibition and measurement of endotoxin of D.p Ag (Dermatophagoides pteronyssinus antigen) 20
4. Quantitation of eotaxins (1,2 and 3), IL-4, IL-5, IL-13, IFN-γ, TGF-β, IL-10 by ELISA 21
5. Intracellular source of eotaxin-2 in total cells (white blood cells) by immunocytochemistry 22
6. Inhibition and blocking study for effect of D.p Ag and LPS 23
7. RNA preparation and Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) 24
8. Statistical analysis 25
III. RESULTS 26
1. Production of eotaxin-1, 2, 3, IL-4, -5, -13, IFN-γ, TGF-β and IL by D.p Ag in peripheral blood cells 26
2. Effect of LPS on eotaxin-2, IL-5, IFN-γ and IL-10 production by peripheral blood cells stimulated with D.p Ag 27
3. Inhibitory effect of LPS on eotaxin-2 and IL-5 production via induction of IL-10 by peripheral blood cells 28
4. Blocking effect of anti-IL-10Rα antibody in LPS induced-attenuation of eotaxin-2 and IL-5 production 29
5. Eotaxin-2 and IL-5 mRNA expression after IL-10Rα blocking 30
6. Blocking effect of anti-TLR4 antibody in LPS induced-attenuation eotaxin-2 and IL-5 30
IV. DISCUSSION 32
References 39
Tables & Figures 54
국문요지 70
감 사 의 글 73
Table 1. Clinical profile and laboratory findings of the study subjects 54
Table 2. Nucleotide sequences of the primers used for RT-PCR 55
Figure 1. Time kinetics and Dose dependent of produced eotaxin-1, 2, 3, IL 4, 5, 13, IFN-γ, TGF-β and IL-10 protein by D.p Ag from D.p Ag positive asthmatics 56
Figure 2-A. Comparison of eotaxin-1, 2, 3, IL-4, 5, 13, IFN-γ, TGF-β and IL-10 by D.p Ag in peripheral blood cells 57
Figure 2-B. Correlation between produced eotaxin-2 level and specific IgE 58
Figure 3. Intra cellular source of eotaxin-2 in peripheral blood cells 59
Figure 4. The effect of dose-dependent with LPS when D.p Ag-induced eotaxin-2 and IL-5 protein in peripheral blood cells of D.p Ag positive subjects 60
Figure 5. Comparison of eotaxin-1,2,3,IL-4,5,13,IFN-γ,TGF-β and IL-10 by LPS in peripheral blood cells 61
Figure 6-A. The inhibitory effects of LPS about D.p Ag-induced eotaxin and IL-5 production in peripheral blood cells 62
Figure 6-B. Dose dependent of produced IFN-γ and IL-10 protein by LPS from D.p Ag positive asthmatics’ peripheral blood cells 63
Figure 7. The inhibitory effects of IFN-γ and IL-10 about D.p Ag eotaxin-2and IL-5 protein in peripheral blood cells of D.p Ag positive subjects 64
Figure 8-A. The blocking effects of IL-10Ra about LPS-induced IL protein in peripheral blood cells of D.p Ag positive subjects 65
Figure 8-B. Gene expression of eotaxin-2 and IL-5 relative to GAPDH in PBMCs 66
Figure 9. The blocking effects of TLR4 about LPS effect in peripheral blood cells of D.p Ag positive subjects 67
Figure 10. Illustrated summary of this study on regulation of eotaxin-2 and IL-5 production by peripheral blood cells 68
Figure 11. Illustrated summary of current and this study on regulation of eotaxin-2 and IL-5 production in monocytes 69
원문구축 및 2018년 이후 자료는 524호에서 직접 열람하십시요.
도서위치안내: / 서가번호:
우편복사 목록담기를 완료하였습니다.
* 표시는 필수사항 입니다.
* 주의: 국회도서관 이용자 모두에게 공유서재로 서비스 됩니다.
저장 되었습니다.
로그인을 하시려면 아이디와 비밀번호를 입력해주세요. 모바일 간편 열람증으로 입실한 경우 회원가입을 해야합니다.
공용 PC이므로 한번 더 로그인 해 주시기 바랍니다.
아이디 또는 비밀번호를 확인해주세요
