표제지
목차
내부연구과제 요약서 [총괄] 3
내부연구과제 연구결과 요약서 [총괄] 4
제1장 연구의 목적 및 필요성 5
제1절 국내외 연구기술개발 현황 5
제2절 연구의 필요성과 중요성 11
제2장 연구의 연구목표 및 달성도, 관련 분야에의 기여도 12
제3장 국내ㆍ외 기술개발 현황 12
제4장 연구의 내용 및 결과 14
제1절 연차별 연구 내용 14
제2절 연차별 연구 방법 및 결과 16
제3절 연차별 연구 결과 요약 45
제5장 연구개발결과의 활용계획 46
제6장 연구의 주요 연구 성과 47
제7장 기대성과 48
제1절 기술적인 측면 48
제2절 경제-산업적 측면 49
제8장 주요 연구 변경사항 49
제9장 연구비 집행 실적 내역 50
제10장 참여연구원 54
제11장 참고문헌 및 첨부문서 54
제1절 참고문헌 54
제2절 첨부문서 58
Table 1/표 13. 위험요인 종류(중복응답) 6
Table 2/Table 1. Prototypical and reference Orientia strains as determined by antigenicity (Kelly DJ et al., Clin Infect Dis 2009, 48:S203-S230) 9
Table 3/Table 2. Selected epidemiolgical prevalence studies showing the geographic distribution of predominant Orientia strains (Kelly DJ et al., Clin Infect Dis 2009, 48:S203-S230) 10
Table 4/Table 3. Primers sequence of INNOPLEX TsuTsu Detection Kit 17
Table 5/Table 4. Primers sequence of 56 kDa gene 19
Table 6/Table 5. Primers sequence of ScaB and ScaD gene 20
Table 7/Table 6. Primers sequence of groEL gene 21
Table 8/Table 7. Demographic characteristics and clinical features of PCR-positive samples. A: Region, B: Gender, C: Age, D: Clinical manifestation 24
Table 9/Table 8. Primers sequence of groEL gene 28
Table 10/Table 9. Primers sequence of 47-kDa gene 30
Table 11/Table 10. Demographic characteristics and clinical features of PCR-positive samples. A: Region, B: Gender, C: Age, D: Clinical manifestation 36
Figure 1. Annual reported cases of scrub typhus in Korea, 2001-2016 6
Figure 2. Circular map of the O. tsutsugamushi genome(Cho NH et al., Proc Natl Acad Sci U S A. 2007. 8;104(19):7981-7986) 13
Figure 3. The result of INNOPLEX TsuTsu Detection Kit. Lane M: 1kb marker, lane 1~15: Inha sample 18
Figure 4. PCR and PCR-RFLP results of 56 kDa gene. Lane M1: 1kb marker, lane M2: 100bp marker, B: Boryong, Kp: Karp, Kt: Kato, G: Gilliam, R.t: R. typhi, R.p: R. prowazaki, P: Pajoo 20
Figure 5. PCR results of ScaB gene and ScaD gene. Lane M1: 1kb marker, lane M2: 100bp marker, B: Boryong, Kp: Karp, Kt: Kato, G: Gilliam, R.t: R. typhi, R.p: R. prowazaki, P: Pajoo 21
Figure 6. PCR results of groEL gene. Lane M: 1kb marker, B: Boryong, Kp: Karp, Kt: Kato, G: Gilliam, R.t: R. typhi 22
Figure 7. The result of INNOPLEX TsuTsu Detection Kit. Lane M: 1kb marker, lane 1~11: samples of suspected scrub typhus patients, N: negative 25
Figure 8. Phylogenetic tree based on partial the 56-kDa type-specific gene sequences. Showing the phylogenetic relationships between O. tsutsugamushi samples in 2014 and the... 27
Figure 9. PCR results of groEL gene. Lane M: 1kb marker, lane 1~10: samples of suspected scrub typhus patients, N: negative 28
Figure 10. Phylogenetic tree based on partial the groEL gene sequences. Samples from the present study are identified by Inha, O. tsutsugamushi from Inha University Hospital;... 29
Figure 11. PCR results of 47-kDa gene. Lane M: 1kb marker, lane 1~4: samples of suspected scrub typhus patients, B: Boryong, N: negative 30
Figure 12. Symptoms and spleen of infected mice. (A) symptoms of infected mice, (B) enlarged spleen of infected mice 31
Figure 13. IFA results of O. tsutsugamushi-infected cells are shown 32
Figure 14. The PCR result (A) and amino acid sequences (B) of 56-kDa gene 33
Figure 15. The result of INNOPLEX TsuTsu Detection Kit. Lane M: 1kb marker, lane 1~10: samples of suspected scrub typhus patients, N: negative 38
Figure 16. Phylogenetic tree based on partial the 56-kDa type-specific gene sequences. Showing the phylogenetic relationships between O. tsutsugamush samples in 2015 and the... 40
Figure 17. Phylogenetic tree based on partial the 56-kDa type-specific gene sequences. Showing the phylogenetic relationships between O. tsutsugamushi samples in 2016 and... 41
Figure 18. IFA results of O. tsutsugamushi-infected cells are shown 43
Figure 19. The PCR result (A) and amino acid sequences (B) of 56-kDa gene 44