Contents
Development and validation of an analytical method to quantify baphicacanthin A by LC-MS/MS and its application to pharmacokinetic studies in mice / So Yeon Jeon ; San Kim ; Jin-Hyang Park ; Im-Sook Song ; Young Taek Han ; Min-Koo Choi 1
Abstract 1
1. Introduction 1
2. Experimental 2
2.1. Chemicals and reagents 2
2.2. Preparation of the stock and working solutions 2
2.3. Preparation of calibration standards and quality control samples 2
2.4. Sample preparation 2
2.5. Instrument conditions 3
2.6. Method validations 3
2.7. Pharmacokinetic experiments 3
2.8. Solubility 4
2.9. Plasma protein binding 4
2.10. Data analysis and statistics 4
3. Results and Discussion 4
3.1. LC-MS/MS analysis of baphicacanthin A 4
3.2. Selectivity and linearity 6
3.3. Precision and accuracy 6
3.4. Extraction recovery and matrix effect 6
3.5. Stability 6
3.6. Pharmacokinetics of baphicacanthin A 6
4. Conclusions 8
References 8
Authors' Positions 9