This study used a modified cetyltrimethylammonium
bromide (CTAB) method to efficiently extract DNA from
the plant pathogenic fungus Cercospora sojina. Total
DNA yield obtained by this method was approximately
1 mg/g of mycelia (fresh weight), and the mean ratio of
A260/A280 and A260/A230 were 2.04 and 2.1, respectively.
The results of random amplified polymorphic
DNA (RAPD) analysis, digestion with restriction enzymes,
and Southern hybridization indicated that polysaccharides
were effectively removed by this method, and the
resulting DNA was sufficient for use in subsequent
molecular analysis.