Repeated treatment of hair permanent wave and coloring cause to hair weakness and cuticle a damage. So that, a optimal treatment condition of hair coloring was proven in this study by different methods. 15 Hair pieces were divided into 3 groups (Room Temperature; RT incubation, heating, ozone treatment). Red and yellow dye were selected for coloring, and hair pieces were treated with color dye in 10 minutes at RT incubation or heating and ozone vapor by using of machine. Value of lightness(L*), redness(a*) and yellowness(b*) were measured for color change observation by chroma meter, and alkaline solubility and swelling were calculated. In the result, ozone treated hair pieces after apply of red and yellow dye were the highest value of L*, a* and b*. And alkaline solubility and swelling by ozone vapor treatment were shown the lowest value. Heating method after apply of red and yellow dye was revealed the lowest value of L*, a* and b*, and the highest level of alkaline solubility and swelling in contrary to ozone vapor treatment. In conclusion, ozone vapor treatment could be useful better than RT incubation and heating for protection of hair damage by coloring, and heating method may be caused hair damage by coloring.