In this study, an analytical method was optimized for detecting dioxin-like PCBs in fish and shellfish. Here,
homogenized samples were extracted using an accelerated solvent extraction (ASE) system with 33 mL cell size. Multilayer
column chromatography, which consisted of acidic, basic, and neutral silica gels, was used for the clean up of the
extracts. The instrumental analysis was executed by HRGC/HRMS to a resolution of 10,000 using 4 window multiple ion
detection (MID) mode. For the results, the average recoveries ranged from 94.1 to 104.1% (?.4) and the limit of detection
was approximately 0.1 pg/g at S/N ratio >3. Finally, the detected concentrations of dioxin-like PCBs for fish and shellfish
were in the range of 0.030-1.836 pg TEQ/g.