Title Page
Contents
Abstract 7
Ⅰ. Introduction 10
Ⅱ. Materials and Methods 14
Plant materials 14
DNA extraction, library preparation, and sequencing 17
Sequence analyses, bioinformatics, and SNP identification 17
Data analyses 18
Ⅲ. Results 20
SNP analysis and genetic diversity 20
Genetic diversity 20
Genetic differentiation and population structure 22
Ⅳ. Discussion 32
Genetic diversity in Z. tyaihyonii populations 32
Genetic differentiation and population structure 35
Implications for the conservation of Z. tyaihyonii 39
Ⅴ. References 43
Abstrcat (in Korean) 58
Table 1. The sampling information of six populations of Zabelia tyaihyonii 16
Table 2. Estimates of genetic diversity within Zabelia tyaihyonii populations. 21
Table 3. Results of analysis of molecular variance (AMOVA) and significance after 20,000 permutations 23
Figure 1. Photos taken during the (A) flowering and (B) fruiting seasons of Zabelia tyaihyonii. 10
Figure 2. Map of sampling sites for this study. 15
Figure 3. The Pairwise-FST values and gene flow (Nₘ) among six populations. The data above the diagonal are Nm; the data below the...[이미지참조] 25
Figure 4. Genetic barriers (red lines) computed from pairwise FST.[이미지참조] 26
Figure 5. Population structure of 94 Z. tyaihyonii individuals from 6 different populations. (A) Relationships between △K and K obtained... 28
Figure 6. Results of principal coordinate analysis (PCoA) of genetic distances for all 94 genotyped individuals. 29
Figure 7. A cladogram of the 94 Zabelia tyaihyonii accessions from six populations. Different colors represent different populations. 31