표제지
목차
Ⅰ. 서론 12
Ⅱ. 재료 및 방법 17
1. 실험 재료 17
(1) 재료 17
(2) 실험 기기 17
(3) 실험 시약 18
2. 추출물 제조 20
(1) 환류 물 추출물 20
(2) 환류 에탄올 추출물 20
3. 실험 방법 22
(1) 유용성분 함량 분석 22
(2) 항산화 활성 24
(3) 항노화 활성 27
(4) 항균활성 29
(5) MTT assay를 이용한 세포독성 효과 30
(6) 항염증 효과 측정 31
4. 통계처리 32
Ⅲ. 결과 및 고찰 33
1. 유용성분 함량 33
(1) 추출수율 33
(2) 환원당 및 수용성 단백질 함량 36
(3) 폴리페놀 및 플라보노이드 화합물 함량 40
2. 항산화 활성 44
(1) ABTS radical cation 소거 활성 44
(2) DPPH radical 소거 활성 47
(3) SOD 유사활성능 50
(4) Xanthine oxidase (XO) 저해 활성 53
(5) 아질산염 소거 활성 55
3. 항노화 활성 62
(1) Collagenase 저해 활성을 통한 항주름 효과 62
(2) Elastase 저해 활성을 통한 항주름 효과 65
(3) Tyrosinase 저해 활성을 통한 미백 효과 68
4. 항균활성 측정 71
5. MTT assay를 이용한 세포독성 및 항염증 효과 측정 73
(1) RAW264.7 대식세포 및 HaCaT 각질형성세포의 세포독성 73
(2) Nitric oxide (NO) 생성 억제능 77
Ⅳ. 적요 80
Ⅴ. 참고문헌 84
초록 99
Abstract 102
Table 1. List of pathogenic bacteria used for Gegeolmu and Raphanus sativus seed water and ethanol extracts. 29
Table 2. Extraction yields of seeds extracts from Gegeolmu and R. sativus 34
Table 3. Contents of the reducing sugar and soluble protein of Gegeolmu and R. sativus seed water and ethanol extacts 37
Table 4. Contents of the total polyphenol and flavonoid of Gegeolmu and R. sativus seed water and ethanol extracts 41
Table 5. ABTS radical scavenging activities of seeds water and ethanol extracts from Gegeolmu and R. sativus 45
Table 6. DPPH radical scavenging ability of seeds water and ethanol extracts from Gegeolmu and R. sativus. 48
Table 7. Superoxide dismutase (SOD) like activity of seeds water and ethanol extracts from Gegeolmu and R. sativus. 51
Table 8. Xanthine oxidase inhibition activity of seeds water and ethanol extracts from Gegeolmu and R. sativus 54
Table 9. Nitrite scavenging ability of seeds water and ethanol extracts from Gegeolmu and R. sativus 58
Table 10. Collagenase inhibition activity of seeds water and ethanol extracts from Gegeolmu and R. sativus 63
Table 11. Elastase inhibition activity of seeds water and ethanol extracts from Gegeolmu and R. sativus 66
Table 12. Tyrosinase inhibition activity of seeds water and ethanol extracts from Gegeolmu and R. sativus 69
Table 13. Antibacterial activities of seeds water and ethanol extracts from Gegeolmu and R. sativus. 71
Table 14. Cell viability of seeds water and ethanol extracts from Gegeolmu and R. sativus on macrophages RAW264.7 and... 74
Table 15. Suppressive effects of seeds water and ethanol extracts from Gegeolmu and R. sativus produced of nitric oxide production... 78
Figure 1. The plant Gegeolmu and Raphanus sativus. 17
Figure 2. Schematic diagram of extract preparation 21
Figure 3. Extraction yields of seeds extracts from Gegeolmu and R. sativus 35
Figure 4. The content of reducing sugar from Gegeolmu and R. sativus seed water and ethanol extracts. Bars with different letters of... 38
Figure 5. The content of soluble protein from Gegeolmu and R. sativus seed water and ethanol extracts. Bars with different letters of... 39
Figure 6. The content of total polyphenol compounds of Gegeolmu and R. sativus seed water and ethanol extracts. Bars with different... 42
Figure 7. The content of total flavonoid compounds of Gegeolmu and R. sativus seed water and ethanol extracts. Bars with different... 43
Figure 8. ABTS radical scavenging ability of seeds water and ethanol extracts from Gegeolmu and R. sativus. Bars with different... 46
Figure 9. DPPH radical scavenging ability of seeds water and ethanol extracts from Gegeolmu and R. sativus. Bars with different... 49
Figure 10. Superoxide dismutase (SOD) like activity of seeds water and ethanol extracts from Gegeolmu and R. sativus. Bars with... 52
Figure 11. Nitrite scavenging abilities of seeds water and ethanol extracts from Gegeolmu and R. sativus at pH 1.2. Bars with different... 59
Figure 12. Nitrite scavenging abilities of seeds water and ethanol extracts from Gegeolmu and R. sativus at pH 3.0. Bars with different... 60
Figure 13. Nitrite scavenging abilities of seeds water and ethanol extracts from Gegeolmu and R. sativus at pH 6.0. Bars with different... 61
Figure 14. Collagenase inhibition activity of seeds water and ethanol extracts from Gegeolmu and R. sativus. Bars with different... 64
Figure 15. Elastase inhibition activity of seeds water and ethanol extracts from Gegeolmu and R. sativus. Bars with different letters of... 67
Figure 16. Tyrosinase inhibition activity of seeds water and ethanol extracts from Gegeolmu and R. sativus. Bars with different... 70
Figure 17. Cell viability of seeds water and ethanol extracts from various parts on macrophages RAW264.7 cells. After RAW264.7 cells (5... 75
Figure 18. Cell viability on HaCaT cells. After HaCaT cells (5 × 10⁴cells) were started in medium for 24h the cells were treated with... 76
Figure 19. Suppressive effects of seeds water and ethanol extracts from Gegeolmu and R. sativus of nitric oixde production from... 79