Title Page
Abstract
Contents
Ⅰ. Introduction 10
Ⅱ. Material and method 14
1. Chemicals and reagents 14
2. Preparation of sample 15
3. LC-MS/MS analysis conditions 17
4. Hair validation study 20
Ⅲ. Result and discussion 22
1. Selectivity 22
2. Linearity 24
3. Accuracy and precision 26
4. Recovery, Process efficiency and matrix effect 28
5. Stability 30
Ⅳ. Conclusion 32
Reference 35
개요 37
Table 1. MRM condition, retention times and condition for THCCOOH, THCCOOH-D₃ 19
Table 2. Calibration curve parameters, LOD and LLOQ for analyte 25
Table 3. Accuracy and precision of THCCOOH 27
Table 4. Recovery, process efficiency, and matrix effect of THCCOOH 29
Table 5. Stability for THCCOOH in hair samples 31
Table 6. Comparison of pretreatment methods and QuEChERS pretreatment 34
Figure1. Blank hair sample (A), THCCOOH standard spike sample (B). 23