As the use of chemicals increases, the global market is gradually expanding due to the specific properties of Nanomaterials (NMs). In korea, as concerns about the exposure of chemicals use to human and the environment increase, The ACT ON REGISTRATION AND EVALUATION OF CHEMICALS amended to create a safe chemical society. Article 16 of the ACT stipulates NMs as substances deemed necessary for hazard assessment and registration is in progress. Concerns about the environmental and human health implications of NMs are growing with the increased use in consumer and industrial products. Given the numerous manufactured NMs already on the market and expected in future, the effort for the individual investigation of hazard and risk would be enormous. To overcome this challenge, grouping of NMs has been identified as one critical issue by the European Commission as well as the Organisation for Economic Co-operation and Development (OECD). The companies that manufacture and import NMs have the advantage of reducing experimental animals and reducing cost and time when building data on materials. The purpose of this research is to investigate the applicability of the NMs grouping scheme in the enforcement of the Act on the Evaluation of Chemical Substances. Research has shown that groups were created as a result of the Ecotox-scheme, a group of aquatic ecotoxicity, to reduce the risk assessment by each property of NMs. In this study was collected physicochemical characteristics by referring to hazardous the aquatic toxicity literature of Algae, Cladocera, Fish for TiO2, ZnO, Ag, CeO2 and CuO NMs. It cannot be grouped by physicochemical characteristics alone, and it has been proved that toxicity result of P.subcapitata in Ag with a sphere shape and citrate coated it was possible to be grouped into one group regardless of size.