표제지
국문초록
목차
Ⅰ. 서론 9
1. 연구의 필요성 및 목적 9
2. 연구가설 13
Ⅱ. 연구방법 14
1. 실험동물 및 사육 14
2. 우울증 유도 동물 모델 14
3. 운동 방법 15
4. 행동 검사 15
1) Tail suspension test 15
2) Forced swim test 16
5. 조직 준비 및 고정 16
6. RNA 추출 16
7. 역전사효소반응 17
8. Real time-PCR 18
9. Immunohistochemistry 18
10. 자료처리방법 19
Ⅲ. 연구결과 20
1. 행동검사 20
1) 집단 간 Tail suspension test 차이 20
2) 집단 간 Forced swim test 차이 21
2. 자발적 휠 러닝 운동량 측정 23
3. 우울증 모델의 병리적 특징 RNA수준의 변화 24
1) 집단 간 등쪽 해마에서 miR-124 발현 수준 차이 24
4. Neurogenesis process와 관련된 단백질 수준 변화 25
1) 집단 간 해마 내 DG에서 Ki67 활성화 수준 차이 25
2) 집단 간 해마 내 DG에서 DCX 활성화 수준 차이 26
3) 집단 간 해마 내 DG에서 NeuN 활성화 수준 차이 27
Ⅳ. 논의 28
1. Tail suspension test 및 Forced swim test 28
2. 자발적 휠 러닝 운동량 측정 변화 29
3. dHP에서 miR-124의 수준 변화 29
4. DG에서 Neuogenesis process의 수준 변화 30
5. 해마에서 miR-124와 Neuogenesis process의 상관관계 31
Ⅴ. 결론 32
참고 문헌 33
ABSTRACT 46
Table 1. 1st Antibody list 19
Table 2. One-way ANOVA for Tail suspension test in each group. 21
Table 3. One-way ANOVA for Forced swim test in each group. 22
Table 4. One-way ANOVA for miR-124 expression level in each group. 24
Table 5. One-way ANOVA for expression level and ratio of Ki67 in each group. 25
Table 6. One-way ANOVA for expression level and ratio of DCX in each group. 26
Table 7. One-way ANOVA for expression level and ratio of NeuN in each group. 27
Figure 1. Procedurse schedule 15
Figure 2. Differences of tail suspension test in each group. (A) Differences of immobility time(sec) in each group. Con : C57BL/6J control, RST : RST control,... 20
Figure 3. Differences of forced swim test in each group. (A) Differences of immobility time(sec) in each group. Con : C57BL/6J control, RST : RST control,... 21
Figure 4. Average of running per 24h over 4 weeks of running wheel acess. (A) Average distance run. (B) Average amount of time spent running. Data represent... 23
Figure 5. Differences of over expression level of miR-124 in dHP in each group. Con : C57BL/6J control, RST : RST control, RST+EXE : RST + VWR exercise,... 24
Figure 6. Differences of expression level of Ki67 in DG in each group. (A) Representative image of Ki67 in DG(40x magnification) in each group. (B) Ki67... 25
Figure 7. Differences of expression level of DCX in DG in each group. (A) Representative image of DCX in DG(40x magnification) in each group. (B) DCX... 26
Figure 8. Differences of expression level of NeuN in DG in each group. (A) Representative image of NeuN in DG(40x magnification) in each group. (B) NeuN... 27