표제지
논문개요
목차
ABBREVIATIONS 13
I. 서론 14
1. 비만의 원인 및 연계 질병의 유병률 14
1-1. Type II Diabetes Mellitus 16
1-2. Hypertension 17
1-3. Hypertriglyceridemia 17
2. 비알코올성 지방간 질환 (Non Alcoholic Fatty Liver Disease; NAFLD) 18
2-1. 원인 18
2-2. 식이 효과 (저탄수화물 고지방식) 20
2-3. 기타 염증인자 간의 관계 20
3. 지방 조직의 염증성 사이토카인 분비 21
4. 연구목적 23
II. 재료 및 방법 24
1. 실험동물 및 식이 24
2. 식이 효율, 체중 증가량 및 식이 섭취량 26
3. 혈액 수치 측정 26
3-1. 혈당 측정 26
3-2. 중성지방 (Triglyceride) 측정 26
3-3. 총콜레스테롤 (Total Cholesterol) 측정 27
4. 간 조직 검사 27
4-1. 독성 검사(AST/ALT) 27
4-2. 간 조직의 지질 추출 (Folch) 28
4-3. 조직검사 (Hematoxylin & Eosin staining) 28
5. 조직 단백질 발현 (Western Blotting analysis) 29
6. Statistical analysis 31
III. 결과 및 고찰 32
1. 체중, 식이 섭취량 및 식이 효율 32
2. 조직 무게 35
3. 간 독성 검사 38
4. Plasma에서의 혈당, 중성지방 및 총콜레스테롤 수치 측정 39
5. 간 조직에서의 중성지방 및 총콜레스테롤 수치 측정 41
6. 간 조직에서의 지방 형성 43
7. 간 조직의 염증성 사이토카인 (Inflammatory Cytokine) 반응 45
8. 지방 조직에서의 Adipogenesis 유전자 발현 47
9. 지방조직에서의 아디포카인(Adipokine) 반응 49
IV. 요약 및 결론 51
참고문헌 53
ABSTRACT 70
Table 1. Diet composition of contents & experiment diets 25
Table 2. 1st antibodies and 2nd antibodies used in western blotting analysis.[이미지참조] 31
Table 3. Weight gain, dietary intake and food efficiency for 2 weeks-treatments with the HF and the Con diets. 34
Table 4. Changes of weights in organs and fat tissues for 2 weeks diets with the HF and the Con. 37
Table 5. Triglyceride and total cholesterol concentration in liver fed HF and Con diets for 2 weeks. 42
Figure 1. The relationships between various biomarkers related to obesity prevalence or obesity-linked disease. 15
Figure 2. The prevalence of obesity and obesity-linked diseases. 16
Figure 3. The transition process spectrum from a healthy liver to hepatocellular carcinoma. 19
Figure 4. Differences in body weight gain (A), dietary intake (B) and food efficiency ratio (C) in the HF and Con groups for 2 weeks. 33
Figure 5. Changes of weights in several organs, such as liver, spleen, kidney and fat tissues (A & B), fed with the HF (60%E)... 36
Figure 6. Changes of activities of plasma AST (A), ALT (B) and AST/ALT ratio (C) in 2 weeks-treatments with the HF (60% E)... 38
Figure 7. Changes of plasma blood glucose (A), triglyceride (B) and total Cholesterol (C) in 2 weeks-treatments with the HF (60%... 40
Figure 8. Triglyceride (A) and total cholesterol (B) concentrations in liver tissues for 2 weeks diet with the HF (60%E... 42
Figure 9. Comparison of H&E staining to find fat accumulation in liver tissues fed the Con (A), the HF for 2 weeks (B) and the HF... 44
Figure 10. Production of proinflammatory cytokines in liver tissues, such as TNF-a and IL-6 in 2 weeks-treatments with... 46
Figure 11. Expression of adipogenesis biomarkers in epi fat such as PPARγ, C/EBPβ fed HF & Con diets for 2 weeks. 48
Figure 12. Expression of inflammatory (leptin) and anti-inflammatory cytokines (adiponectin) and their ratio in fat tissues in 2 weeks diet... 50