표제지
ABSTRACT
목차
기호 및 약어 설명 8
제1장 서언 9
제2장 재료 및 방법 11
1절 식물 재료 11
2절 흰가루병 병원균 동정 및 접종 13
1. 광학현미경을 통한 흰가루병 균주의 형태학적 관찰 13
2. PCR & 젤 전기영동 밴드 확인을 통한 흰가루병 병원균 동정 13
3. 흰가루병 접종 방법 15
3절 흰가루병 저항성 표현형 평가 및 선발 16
4절 흰가루병 저항성 분자 마커를 이용한 유전형 평가 및 선발 17
1. gDNA 추출 및 concentration 확인 17
2. 흰가루병 저항성 프라이머 선정 17
3. PCR 및 젤 전기영동법을 통한 band 확인 및 선발 18
5절 흰가루병 저항성 평가 결과 비교 18
제3장 결과 19
1절 애호박 계통 60점에 대한 잎 특성 조사 19
2절 흰가루병 병원균 동정 22
1. 흰가루병 균주의 형태학적 관찰을 통한 동정 22
2. 흰가루병 균주의 형태학적 관찰을 통한 동정 22
3. PCR 및 젤 전기영동 밴드 확인을 통한 흰가루병 병원균 동정 24
3절 흰가루병 저항성 표현형 평가 결과 26
1. 흰가루병 접종 후 병 발생 실태 26
2. 흰가루병 병반면적률(%)에 따른 표현형 검정 결과 26
3. 흰가루병 저항성 표현형 평가에 따른 저항성 계통 선발 34
4절 흰가루병 저항성 유전형 평가 결과 35
1. gDNA 추출 및 concentration 확인 35
2. PCR 및 젤 전기영동을 통한 흰가루병 저항성 검정 36
3. 표현형 검정 및 유전형 평가 결과 비교 36
제5절 흰가루병 저항성 계통 최종 선발 44
제4장 고찰 45
제5장 결론 47
참고문헌 48
요약문 51
Table 1. Primers for Powdery mildew pathogen detection. 14
Table 2. Primers for powdery mildew resistance genotype test. 18
Table 3. Grade on leaf characteristics of C. Moschata breeding lines(JNCM-001-030). 20
Table 4. Grade on leaf characteristics of C. Moschata breeding lines(JNCM-031-060). 21
Table 5. Results of resistance determination based on the percentage of powdery mildew lesion area. 33
Table 6. List of selected BN through phenotype test. 34
Table 7. Measurement value of gDNA concentration. 35
Table 8. Degree of resistant concordance between genotype test results for phenotype test results. 36
Table 9. Result for genotype test of powdery mildew resistance. 43
Table 10. List of final selected breeding lines for powdery mildew resistance. 44
Figure 1. Overall cultivation management of C. Moschata. (A) Management of temperature and humidity of nursery facilities. (B) The average number of leaves at transplantation. (C) Rope... 12
Figure 2. Method to inoculate powdery mildew. (A) Spray inoculation method. (B) compressed inoculation method. 15
Figure 3. Phenotype test stage according to the incidence area ratio(%). (A) 0 stage; incidence area ratio: 0%, (B) 1 stage; incidence area ratio: 1~5%, (C) 2 stage; incidence area ratio: 6~10%, (D) 3... 16
Figure 4. Leaf characteristic research standard of C. Moschata. (A) plant vigor (B) size of a leaf body (C﹚ length of a petiole (D) angle of the edge of a leaf body (E﹚ Green strength of the leaf body (F)... 19
Figure 5. Sign of powdery mildew on C. Moschata leaves. (A) Anseong (B) Pyeongtaek (C) Jincheon (D) Osan. 22
Figure 6. Shape of powdery mildew fungi spores on C. Moschata leaves. (A) Powdery mildew spores collected in Anseong (B) Powdery mildew spores collected in Pyeongtaek (C) Powdery mildew spores... 23
Figure 7. Result of PCR identification for powdery mildew pathogen(Podosphaera xanthii). using primer : PxK F/R. (A) powdery mildew fungi gDNA collected in Anseong, 1&2 repeat (B) Powdery... 24
Figure 8. Result of PCR identification for powdery mildew pathogen(Podosphaera xanthii). using primer : S1/S2. (A) powdery mildew fungi gDNA collected in Anseong, 1&2 repeat (B) Powdery... 25
Figure 9. Result of PCR identification for powdery mildew pathogen(Golovinomyce cichoracearum). using primer : G1/G2 (A) powdery mildew fungi gDNA collected in Anseong, 1&2 repeat (B)... 25
Figure 10. Number of infected BN after inoculation of powder. 26
Figure 11. Result of survey on the percentage of powdery mildew lesion area (JMCM 1~10) 27
Figure 12. Result of survey on the percentage of powdery mildew lesion area (JMCM 11~20) 28
Figure 13. Result of survey on the percentage of powdery mildew lesion area (JMCM 21~30) 29
Figure 14. Result of survey on the percentage of powdery mildew lesion area (JMCM 31~40) 30
Figure 15. Result of survey on the percentage of powdery mildew lesion area (JMCM 41~50) 31
Figure 16. Result of survey on the percentage of powdery mildew lesion area (JMCM 51~60) 32
Figure 17. Result of PCR with powdery mildew resistance primer(PMR1). 37
Figure 18. Result of PCR with powdery mildew resistance primer(PMR1). 38
Figure 19. Result of PCR with powdery mildew resistance primer(PMR2). 39
Figure 20. Result of PCR with powdery mildew resistance primer(PMR2). 40
Figure 21. Result of PCR with powdery mildew resistance primer(PMR5). 41
Figure 22. Result of PCR with powdery mildew resistance primer(PMR5). 42