표제지
ABSTRACT
목차
제1장 서론 8
제2장 연구사 11
1. 고양이 11
2. 반려동물 13
3. 반려동물 산업 20
4. 유전자 분석을 통한 개체식별 시스템 22
5. 개체식별마커 24
제3장 재료 및 방법 26
1. 공시재료 26
2. Genomic DNA Extraction 및 QC 28
3. PCR 28
4. 유전자형 분석 30
5. 통계분석 30
제4장 결과 31
1. 유전자형 분석 결과 31
2. Microsatellite Marker 다형성 분석 45
3. 주성분분석 (Principal Coordinates Analysis, PCoA) 51
4. 요인대응분석 (Factorial Correspondence Analysis, FCA) 53
5. 집단 간 유전거리 추정 55
6. 집단의 유전적 균일도 추정 56
제5장 고찰 57
참고문헌 60
국문요약 69
Table 1. Comparison of domestic companion animal raising rates by each survey institution 15
Table 2. Comparison of companion animal registration systems in each country 19
Table 3. Sample information used in this study 26
Table 4. Primer information for 11 selected microsatellite markers in the study 29
Table 5. Number of allele to 10 selected microsatellite markers 31
Table 6. Number of allele to FCA220 Marker 32
Table 7. Number of allele to FCA069 Marker 34
Table 8. Number of allele to FCA453 Marker 35
Table 9. Number of allele to FCA075 Marker 37
Table 10. Number of allele to FCA229 Marker 38
Table 11. Number of allele to FCA149 Marker 39
Table 12. Number of allele to FCA105 Marker 40
Table 13. Number of allele to FCA649 Marker 41
Table 14. Number of allele to FCA026 Marker 43
Table 15. Number of allele to FCA293 Marker 44
Table 16. Na, Hexp, Hobs and PIC observed across 10 MS markers[이미지참조] 45
Table 17. Na, Hexp, Hobs and PIC of 10 microsatellite loci for 10 breed cat (KS, TA, PS)[이미지참조] 48
Table 18. Na, Hexp, Hobs and PIC of 10 microsatellite loci for 10 breed cat (SM, RB, SF)[이미지참조] 49
Table 19. Na, Hexp, Hobs and PIC of 10 microsatellite loci for 10 breed cat (MC, BG, BS, MIX)[이미지참조] 50
Figure 1. Status of domestic companion animal raising by year 14
Figure 2. Proportion of raising by dog and cat breed 16
Figure 3. Status of abandoned animals by domestic companion animal breed 18
Figure 4. Reasons for non-registration of animal registration system (2021) 19
Figure 5. Increase in the number of veterinary clinics and veterinarians (2018-2021) 21
Figure 6. Schematic diagram of the production traceability system for Hanwoo 23
Figure 7. Photos by cat breed 27
Figure 8. 212 allele of FCA220 Marker 33
Figure 9. 216 allele of FCA220 Marker 33
Figure 10. 187 allele of FCA453 Marker 36
Figure 11. 146 allele of FCA649 Marker 42
Figure 12. PCoA of allele frequencies from 10 MS markers typed in 10 population using the GenAlEx 6.4. 51
Figure 13. FCA of allele frequencies from 10 MS markers typed in 10 population using the GENETIX ver. 4.05. 53
Figure 14. POPTREE2 show the genetic distances among the 10 breeds on the allele frequencies from 10 microsatellite loci 55
Figure 15. Plot of genomic components estimated for 10 breeds sample using the software STRUCTURE ver. 2.3.3 from K=2 to K=10 56