Title Page
ABSTRACT
Contents
Ⅰ. INTRODUCTION 11
Ⅱ. MATERIAL AND METHODS 13
1. Materials 13
2. Isolation and purification of Aloe-derived Nanovesicles 13
3. Cell cultures 13
4. Nanoparticle tracking analysis and TEM analysis 14
5. DiR-DAPI staining 14
6. Biodistribution targeting of orally administration aloe-derived nanovesicles 15
7. Animal treatments 15
8. Histological analysis of the colon and small intestine 16
9. Immunoblot analysis 16
10. Endotoxin assay 17
11. Enzyme-Linked Immunosorbent Assay (ELISA) 17
12. Cell viability 18
13. Permeability (TEER and FITC) 18
14. Statistical analysis 19
Ⅲ. RESULTS 20
1. VNVs, SNVs, and ANVs were purified and characterized 20
2. In vitro, In vivo uptake of aloe-derived nanovesicles 22
3. VNVs, SNVs, and ANVs alleviated the disease severity in DSS-induced acute colitis mice 24
4. VNVs, SNVs, and ANVs alleviated the disease severity and restored TJ/AJ in DSS-induced acute colitis mice colon 27
5. VNVs, SNVs, and ANVs alleviated the TJ and AJ in DSS-induced acute colitis mice small intestine 30
6. VNVs, SNVs, and ANVs enhanced epithelial barrier function in T84 cells 33
7. Enhancement of TJ/AJ of VNVs in colorectal cancer cells, HT-29. 35
Ⅳ. DISCUSSION 37
Ⅴ. REFERENCES 41
ABSTRACT IN KOREAN 46
Table 1. Scoring system to calculate the disease activity index (DAI). 25
Figure 1. Characterization of VNVs, ANVs, and SNVs. 21
Figure 2. in vitro, in vivo uptake of aloe-derived nanovesicles. 23
Figure 3. VNVs, ANVs, and SNVs attenuated the disease severity and levels of plasma Endotoxin, NO, and TNF-α in DSS-induced acute colitis. 26
Figure 4. VNVs, ANVs, and SNVs prevents colon damage in mice with DSS-induced acute colitis. 28
Figure 5. VNVs, ANVs, and SNVs prevents small intestine damage in mice with DSS-induced acute colitis. 31
Figure 6. VNVs, AN Vs, and SNVs protected the levels of TJ proteins and reduces of permeability in DSS-induced human colon cancer-derived cell... 34
Figure 7. VNVs protected the levels of TJ/AJ proteins in DSS-induced human colon cancer-derived cell line HT-29. 36
Figure 8. Schematic illustrations to describe a therapeutic mechanism of aloe-derived nanovesicles on intestinal epithelial damage caused by... 38