표제지
목차
Ⅰ. 서론 12
1. 연구 배경 및 필요성 12
1.1. 연구 대상 소재 12
2. 목표 효능 17
2.1. 이너뷰티 기능성 17
2.2. 항염증 18
2.3. 피부 미백 21
2.4. 주름 개선 24
2.5. 항비만 25
3. 연구 목적 28
Ⅱ. 재료 및 방법 29
1. 실험재료 29
1.1. 시료 29
1.2. 시약 및 기기 32
2. 실험방법 34
2.1. 시료 제조 및 추출 34
2.2. 기능 성분 함량 분석 및 항산화 활성 평가 36
2.3. 녹차 추출물별 Catechins 정량분석 37
2.4. 이너뷰티 기능성 관련 in vitro 효능평가 39
2.5. 콤부차에 대한 성분분석 및 이화학적 특성 평가 55
2.6. 콤부차에 대한 기능성 평가 58
2.7. 통계 분석 58
Ⅲ. 결과 및 고찰 59
Part 1. 녹차 추출물과 기능성 강화 및 당 보강 소재 효능평가 59
1. 녹차 추출물별 기능성 효능평가 및 성분분석 59
2. 기능성 강화 및 당 보강 소재 효능평가 및 우수소재 효능작용기전 구명 72
Part 2. 콤부차 개발 및 기능성 효능평가 97
1. 콤부차에 대한 성분분석 및 이화학적 특성 평가 97
2. 콤부차에 대한 기능성 평가 102
Ⅳ. 요약 및 결론 108
1. 녹차 추출물 중 우수 추출물 선정 ; GWE의 효능 검증 및 성분분석 108
2. 천연물 자원 중 기능성 우수소재 선정 ; MCE의 이너뷰티 기능성 효능 검증 109
3. 박하 추출액 함유 콤부차(MK) 제조 및 효능 검증 110
Ⅴ. 참고문헌 111
Abstract 119
Table 1. List of Publications of Kombucha used in the previous study 14
Table 2. List of Patents of Kombucha used in the previous study 15
Table 3. List of Resources 31
Table 4. HPLC condition of catechins in the HPLC analysis 38
Table 5. Anti-inflammatory primer sequences used for qRT-PCR 41
Table 6. Anti-inflammatory antibody used in western blot analysis 43
Table 7. Whitening primer sequences used for qRT-PCR 46
Table 8. Whitening antibody used in western blot analysis 48
Table 9. Anti-obesity Primer sequences used for qRT-PCR 52
Table 10. Anti-obesity Antibody used in western blot analysis 54
Table 11. HPLC condition of organic acids in the HPLC analysis 56
Table 12. Extraction yield, total polyphenol contents and antioxidant activity of green tea extracts 60
Table 13. Composition of Catechins in green tea extracts. 62
Table 14. Composition of organic acids in Kombucha 98
Table 15. Comparison of pH, acidity, sugar contents and chromaticity between K and MK 101
Fig. 1. Mechanism of inflammation in macrophage 20
Fig. 2. Synthesis of melanin and representation of the functions of involved enzymes 23
Fig. 3. The mechanism of cell differentiation in adipocyte 27
Fig. 4. The procedure for the extraction 35
Fig. 5. The Chromatogram of HPLC for catechins and green tea extracts analysis. 63
Fig. 6. Effect of green tea extracts on cell viability(A) and nitric oxide product level(B) in LPS-induced RAW264.7 cells. 65
Fig. 7. Effect of green tea extracts on cell viability(A) and melanin content(B) in α-MSH-induced B16F10 cells. 67
Fig. 8. Effect of Green tea extracts on cell viability(A) and skin regeneration(B) in HS68 cells. 69
Fig. 9. Effect of Green tea extracts on cell viability(A) and Lipid accumulation(B) in 3T3-L1 cells. 71
Fig. 10. Effect of natural material 70% ethanol extracts on cell viability(A) and nitric oxide product level(B) in LPS-induced RAW264.7 cells. 74
Fig. 11. Effect of natural material 70% ethanol extracts on cell viability(A) and melanin content(B) in α-MSH-induced B16F10 cells. 77
Fig. 12. Effect of natural material 70% ethanol extracts on cell viability(A) and skin regeneration(B) in HS68 cells. 80
Fig. 13. Effect of natural material 70% ethanol extracts on cell viability(A) and Lipid accumulation(B) in 3T3-L1 cells. 83
Fig. 14. Effect of Mentha canadensis 70% ethanol extract(MCE) on cell viability(A) and NO product level(B) in LPS-induced RAW264.7 cells. 85
Fig. 15. Effect of Mentha canadensis 70% ethanol extract(MCE) on the mRNA(A,B) and protein(C,D) expressions of iNOS and COX-2 in LPS-induced... 87
Fig. 16. Effect of Mentha canadensis 70% ethanol extract(MCE) on cell viability(A), melanin contents(B) and tyrosinase inhibitory(C) in B16F10 cells. 89
Fig. 17. Effect of Mentha canadensis 70% ethanol extract(MCE) on the protein(A-C) and mRNA(D-F) expressions of Tyrosinase, TRP1 and TRP2 in... 91
Fig. 18. Effect of Mentha canadensis 70% ethanol extract(MCE) on cell viability(A) and lipid accumulation(B) in 3T3-L1 cells. 93
Fig. 19. Effect of Mentha canadensis 70% ethanol extract(MCE) on the protein(A-E) and mRNA(F-J) expressions of PPARγ, SREBP-1C, C/EBPα,... 96
Fig. 20. The Chromatogram of HPLC for organic acids, FMK and MK analysis. 99
Fig. 21. Effect of kombucha on cell viability(A) and NO product level(B) in LPS-induced RAW264.7 cells. 103
Fig. 22. Effect of kombucha on cell viability(A) and melanin contents(B) in α-MSH-induced B16F10 cells. 105
Fig. 23. Effect of kombucha on cell viability(A) and lipid accumulation(B) in 3T3-L1 cells. 107