표제지
목차
Abstract(초록) 9
Ⅰ. 서론 12
Ⅱ. 재료 및 방법 19
1. 시약 및 항체 정보 19
2. 겨우살이 물 추출물 제조 20
3. 세포 배양 21
4. 세포 생존율 측정 21
5. MDA-MB-231/THP-1 공동배양 모델 23
6. Wound healing 측정 25
7. Transwell migration 측정 25
8. Enzyme-linked immunosorbent assay (ELISA) 27
9. Cytokine array 27
10. Western blot 28
11. Immunofluorescence 30
12. 통계 분석 31
Ⅲ. 결과 32
1. MDA-MB-231 및 THP-1 세포에 대한 국내산 겨우살이 물 추출물의 세포 생존율 측정 32
2. 삼중음성유방암 세포 MDA-MB-231의 이동성 저해 효과 측정 34
3. 염증성 사이토카인 분비에 미치는 효과 39
4. Apoptosis 관련 단백질 발현에 미치는 효과 44
5. STAT3 단백질 활성화 저해 효과 53
6. MMPs 발현 저해에 미치는 효과 56
7. EMT marker 발현에 미치는 영향 61
Ⅳ. 고찰 64
References 77
영문 초록 95
Fig. 1. MDA-MB-231/THP-1 M0 macrophage co-culture model using transwell system. THP-1 monocytes were differentiated with PMA (10 ng/mL) for 48 h before plated in... 24
Fig. 2. Cytotoxic effect of mistletoe water extracts in (A) MDA-MB-231 and (B) THP-1 cells for 24 h and 48 h. Each cell line was treated with various concentration of... 33
Fig. 3. Effects of Korean mistletoe water extracts on wound healing assay in MDA-MB-231 and MDA-MB-231/THP-1 co-culture cells. After scratch on both cell groups,... 37
Fig. 4. Effect of Korean mistletoe water extracts on transwell migration assay in (A) MDA-MB-231 and (B) MDA-MB-231/THP-1 co-culture cells. Mistletoe water extracts... 38
Fig. 5. Effects of mistletoe water extracts on pro-inflammatory cytokine (IL-6) production in LPS-stimulated MDA-MB-231 and MDA-MDA-MB-231/THP-1 co-... 41
Fig. 6. Effects of Korean mistletoe water extracts on (A) IL-4, (B)TGF-β and (C) TNF-γ secretion in MDA-MB-231 and MDA-MB-231/THP-1 co-culture cells. Both cell... 43
Fig. 7. Effects of Korean mistletoe water extracts on apoptosis-related protein expression in MDA-MDA-231 and MDA-MB-231/THP-1 co-culture cells using... 48
Fig. 8. Representative images of pro-apoptosis proteins and their quantitative bar graph using immunofluorescence staining. MDA-MB-231 and MDA-MB-231/THP-1 co-... 51
Fig. 9. Inhibitory effects of Korean mistletoe water extracts on STAT3 activation in MDA-MB-231 and MDA-MB-231/THP-1 co-culture cells. After treatment with 0, 0.1,... 55
Fig. 10. Inhibitory effects of MMP-2 and MMP-9 expression in MDA-MB-231 and MDA-MB-231/THP-1 co-culture cells. Both groups were treated with mistletoe water... 58
Fig. 11. Representative images from immunofluorescence staining of (A) MMP-2 and (B) their quantitative bar graph. MDA-MB-231 and MDA-MB-231/THP-1 co-culture... 59
Fig. 12. Effects of Korean mistletoe water extracts on regulating EMT markers in MDA-MB-231 and MDA-MB-231/THP-1 co-culture cells using western blot analysis. Both... 63