표제지
목차
Abbreviations 9
I. 서론 10
II. 재료 및 방법 13
1. 재료 13
1) 실험동물 13
2) 약물의 조제 13
2. 방법 13
1) 실험군의 분리 13
2) 만성 알코올성 근손상 유발과 약물 처치 14
3) 체중 측정 14
4) 근육 중량의 변화 측정 15
5) 해부조직학적 관찰 및 근섬유 단면적 측정 15
6) 면역조직화학염색 15
7) 통계 분석 16
III. 결과 17
1. 체중의 변화 17
2. 근육 중량의 변화 19
3. 근육 단면적의 해부조직학적 변화 23
4. 근섬유 단면적 크기비교 27
5. 면역조직화학적 관찰 30
1) TNF-α 30
2) IL-6 33
3) IL-1β 36
4) BAX 39
5) Bcl-2 42
IV. 고찰 46
V. 결론 54
Abstract 56
참고문헌 58
Table 1. Muscle Weights of Gastrocnemius, Soleus and Plantaris in Rats after 6 Weeks. 22
Table 2. Comparison of Cross-Sectional Area of Gastrocnemius, Plantaris and Soleus in Rats after 6 Weeks. 29
Table 3. Comparison of BAX / Bcl-2 Ratio of Normal, EtOH and EtOH+RVS Groups in Rats after 6 Weeks. 45
Figure 1. Changes of body weights in Normal, EtOH, and EtOH+RVS(Rhus Verniciflua Stokes; RVS) groups. 18
Figure 2. Representative comparison of leg muscle(gastrocnemius) after 6 weeks. 20
Figure 3. Muscle weights of gastrocnemius, soleus and plantaris in rats after 6 weeks. 21
Figure 4. Representative hematoxylin & eosin staining of gastrocnemius muscle in rats after 6 weeks(magnification×400). 24
Figure 5. Representative hematoxylin & eosin staining of soleus muscle in rats after 6 weeks(magnification×400). 25
Figure 6. Representative hematoxylin & eosin staining of plantaris muscle in rats after 6 weeks(magnification×400). 26
Figure 7. Cross-sectional area of muscle fiber of gastrocnemius, soleus and plantaris muscles in rats. 28
Figure 8. Representative photos of TNF-α immunoactivities of gastrocnemius in rats after 6 weeks. 31
Figure 9. TNF-α immunoactivities of gastrocnemius muscles in rats after 6 weeks. 32
Figure 10. Representative photos of IL-6 immunoactivities of gastrocnemius muscles in rats after 6 weeks. 34
Figure 11. IL-6 immunoactivities of gastrocnemius muscles in rats after 6 weeks. 35
Figure 12. Representative photos of IL-1β immunoactivities of gastrocnemius muscles in rats after 6 weeks. 37
Figure 13. IL-1β immunoactivities of gastrocnemius muscles in rats after 6 weeks. 38
Figure 14. Representative photos of BAX immunoactivities of gastrocnemius muscles in rats after 6 weeks. 40
Figure 15. BAX immunoactivities of gastrocnemius muscles in rats after 6 weeks. 41
Figure 16. Representative photos of Bcl-2 immunoactivities of Gastrocnemius muscles in Rats after 6 weeks. 43
Figure 17. Bcl-2 immunoactivities of gastrocnemius muscles in rats after 6 weeks. 44