표제지
목차
I. 서론 10
II. 재료 및 방법 12
1. 재료 12
1) 동물 12
2) MC3T3-E1 세포 13
2. 방법 13
1) 골절 모델 13
2) 골 유합 효능 검사 17
3) 조골세포 분화능 검사 19
4) 안전성 검사 20
3. 통계분석 22
III. 결과 23
1. 골 유합 효능 분석 23
1) 형태학적 분석 23
2) 조직학적 분석 25
3) 골강도 분석 31
2. 조골세포 분화능 분석 35
1) ALP 염색 및 활성도 35
2) Alizarin Red S 염색 및 골 석회화 형성도 37
3. 안전성 분석 39
1) 세포 독성 39
2) 간기능 41
3) 신기능 43
IV. 고찰 45
V. 결론 50
참고문헌 51
ABSTRACT 57
Fig. 1. The surgical procedure for the femur-fractured mouse model. 14
Fig. 2. Experimental design of this study. 16
Fig. 3. Morphological analysis of femur fracture bone by visual observation and X-ray observation. 24
Fig. 4. Hematoxylin & Eosin staining of femur fracture bone. 26
Fig. 5. Safranin O staining of femur fracture bone. 28
Fig. 6. Masson's trichrome staining of femur fracture bone. 30
Fig. 7. Effect of BPXplus on tensile force of bone after femur fracture.[이미지참조] 32
Fig. 8. Effect of BPXplus on compressive force of bone after femur fracture.[이미지참조] 34
Fig. 9. Effect of BPXplus on osteoblast differentiation observed by ALP staining in MC3T3-E1 cells.[이미지참조] 36
Fig. 10. Effect of BPXplus on osteoblast differentiation and bone calcification formation observed by Alizarin Red S staining in MC3T3-E1 cells.[이미지참조] 38
Fig. 11. Effect of BPXplus on cell viability in MC3T3-E1 cells.[이미지참조] 40
Fig. 12. Effect of BPXplus on the level of ALT, AST, and LDH in plasma.[이미지참조] 42
Fig. 13. Effect of BPXplus on the level of BUN, Creatinine in plasma.[이미지참조] 44