Title Page
Contents
INTRODUCTION 7
METHODS 10
Animal 10
Tissues & body fluids 10
Reagents 10
Cell counting 11
Sepsis models 11
Measurement of cytokine levels 11
Determination of colony-forming units (CFUs) 12
Statistical analysis 12
RESULTS 13
LPA protects against sepsis-induced lethality 13
Effect of 18:1 LPA on various cytokine levels in peritoneal lavage fluid in response to CLP. 14
LPA enhances bacterial clearance 14
LPA reduces sepsis model-induced accumulation of neutrophils 15
LPA recovers sepsis-induced multiple-organ dysfunction 15
LPA changes the blood-cell ratio in naïve mouse 16
DISCUSSION 24
REFERENCE 27
Abstract 31
Fig.1. LPA protects against sepsis-induced lethality. 18
Fig.2. LPA changes cytokine levels in peritoneal lavage fluids of sepsis model mice. 19
Fig.3. LPA enhances bacterial clearance. 20
Fig.4. LPA reduces sepsis model-induced accumulation of neutrophils. 21
Fig.5. LPA recovers sepsis-induced multiple organ dysfunction. 22
Fig.6. LPA changes blood-cell ratio in naïve mouse. 23