표제지
목차
국문초록 13
I. 서론 16
1. 연구의 필요성 16
2. 연구의 목적 24
II. 연구 방법 25
1. 샘플 사이즈 계산 및 시편 제작 27
2. 구강 미세생태계 바이오필름의 형성 29
3. 항균 처치 31
4. 구강 미세생태계 바이오필름의 붉은형광강도 분석 36
5. 생균 집락 수 평가 38
6. 세균 생존율 분석 40
7. 통계분석 42
III. 연구 결과 43
1. 초기 구강 미세생태계 바이오필름에 대한 저온 대기압 플라즈마의 항균력 43
1) 초기 바이오필름의 붉은형광강도 43
2) 초기 바이오필름의 생균 집락 수 48
3) 초기 바이오필름의 세균 생존율 51
2. 후기 구강 미세생태계 바이오필름에 대한 저온 대기압 플라즈마의 항균력 53
1) 후기 바이오필름의 붉은형광강도 53
2) 후기 바이오필름의 생균 집락 수 58
3) 후기 바이오필름의 세균 생존율 61
IV. 고찰 63
V. 결론 71
VI. 참고문헌 74
Abstract 84
Table 1. The mean △RatioR/G of young biofilm according to the treatment process[이미지참조] 46
Table 2. The mean total bacteria and aciduric bacteria of young biofilm 49
Table 3. The mean △RatioR/G of old biofilm according to the treatment process[이미지참조] 56
Table 4. The mean total bacteria and aciduric bacteria of old biofilm 59
Figure 1. Schematic diagram of the study procedure (CFUs, Colony forming units; CHX, Chlorhexidine; CLSM, confocal laser scanning microscopy; DW, distilled water;... 26
Figure 2. Preparation of specimens for this experiment (a), Preparation of HA disks (7 ㎜ x 2 ㎜); (b), HA disks are embedded in an acrylic mold with a space of... 28
Figure 3. The treatment process of dental microcosm biofilm Initially, the HA disk in which the biofilm is formed is placed on the first row of 24-well cell culture... 33
Figure 4. Plasma pipette® (a), The parts of the plasma pipette® ; (b), A length of the white dotted line (10 ㎜) indicates the distance between the tip of the plasma flame...[이미지참조] 34
Figure 5. Flow diagram of the maturation and treatment procedures in Study 1 and 2 (BMM, basal medium mucin; CHX, Chlorhexidine; DW, distilled water;... 35
Figure 6. Measurement of red and green intensity for RatioR/G analysis using Image PRO® RatioR/G is calculated as red intensity/green intensity. (ROI, Region...[이미지참조] 37
Figure 7. Live/dead assay procedure of biofilm suspension (a), Equal volumes of live/dead dye solutions are combined and 3 ㎕ of the mixture is added to the bacterial... 41
Figure 8. Differences in RatioR/G depending on treatment methods and period of young biofilm Data are presented as mean. a,b,cDifferent letters within...[이미지참조] 44
Figure 9. Red fluorescence image of young biofilm formed on the HA disks The number in the lower right presents RatioR/G. (a), (b), (c) and (d), After DW...[이미지참조] 47
Figure 10. Representative images of CFUs according to the treatment process on young biofilm (a), 10-5 DW; (b), 10-5 CHX; (c), 10-5 NTAPPJ; (d), 10-3...[이미지참조] 50
Figure 11. Representative CLSM fluorescent images (x100) showing the live (green) and dead (red) stained young biofilm bacteria adherent to... 52
Figure 12. Differences in RatioR/G depending on treatment methods and period on old biofilm Data are presented as mean. a,b,cDifferent letters within the...[이미지참조] 54
Figure 13. Red fluorescence image of old biofilm formed on the HA disks The number in the lower right presents RatioR/G. (a), (b), (c) and (d), After DW...[이미지참조] 57
Figure 14. Representative images of CFUs according to the treatment process on old biofilm (a), 10-5 DW; (b), 10-5 CHX; (c), 10-5 NTAPPJ; (d), 10-3 DW;...[이미지참조] 60
Figure 15. Representative CLSM fluorescent images (x100) showing the live (green) and dead (red) stained old biofilm bacteria adherent to HA... 62