Title Page
Contents
ABSTRACT 9
I. Introduction 11
II. Materials and Methods 13
1. Reagents 13
2. Methods 14
2.1. In vitro study 14
2.2. In vivo study 21
III. Results 23
1. Anti-oxidant, anti-bacterial and anti-inflammatory activity 23
2. Protein components 27
3. 5α-reductase inhibition 30
4. Proliferation and migration of HFDPC and HaCaT 32
5. Expression of Wnt/ β-Catenin, VEGF, Bcl-2 and Bax in HaCaT cells 35
6. In vivo hair growth promotion 39
7. Histopathological staining 41
IV. Discussion 43
V. References 47
국문요약 54
Table 1. Anti-oxidative activity of sturgeon mucus. 25
Table 2. Protein components of sturgeon mucus via 2D/LC-MS/MS. 29
Figure 1. Anti-bacterial and anti-inflammatory activity of sturgeon mucus. 26
Figure 2. 2-dimensional electrophoretic map of sturgeon mucus following 2D at non-linear pH 3.0 - 10.0 28
Figure 3. Inhibitory effect of sturgeon mucus on male hormone. 31
Figure 4. Cell proliferation promoting activity of sturgeon mucus. 33
Figure 5. Wound healing activity of sturgeon mucus. 34
Figure 6. Activation of Wnt/ β-catenin signaling by sturgeon mucus. 36
Figure 7. Activation of VEGF expression by sturgeon mucus. 37
Figure 8. Effect of sturgeon mucus on the expression of apoptotic proteins. 38
Figure 9. Analysis of in vivo hair growth promotion. 40
Figure 10. Representative histological observation of hair growth in C57BL/6N mice. 42