표제지
목차
국문요약 8
I. 서론 10
II. 재료 및 방법 13
1. 시약 13
2. 세포배양 13
3. 세포 독성 평가 13
4. RNA 추출 및 Real time RT-PCR 14
5. Western blot을 이용한 단백질 발현 분석 15
6. 통계 처리 15
III. 결과 18
1. Taxifolin의 안전성 평가 18
2. TNF-α가 유도된 염증 환경에서 탁시폴린에 의한 사이토카인 발현 조절 20
3. TNF-α가 유도된 염증 환경에서 탁시폴린에 의한 케모카인 발현 조절 22
4. TNF-α 유도 조건에서 탁시폴린에 의한 IκB 단백질 인산화 조절 24
5. TNF-α 유도 조건에서 탁시폴린에 의한 MAPK 신호 단백질 인산화 조절 25
6. TNF-α 유도 조건에서 탁시폴린에 의한 STAT3 단백질 인산화 조절 28
7. TNF-α/IL-17A/IFN-γ 환경에서 탁시폴린에 의한 사이토카인 발현 조절. 30
8. TNF-α/IL-17A/IFN-γ 환경에서 탁시폴린에 의한 케모카인 발현 조절 32
9. TNF-α/IL-17A/IFN-γ 환경에서 IκB 단백질 인산화 조절 34
10. TNF-α/IL-17A/IFN-γ 환경에서 MAPK 단백질 발현 조절 36
11. TNF-α/IL-17A/IFN-γ 환경에서 STAT3 단백질 인산화 조절 38
IV. 결론 40
V. 참고문헌 42
Appendix 47
Abstract 48
Table 1. Probes used for real time RT-PCR 16
Table 2. Antibody list used in western blot analysis 17
Fig. 1. (A) Structure of taxifolin. (B) The cytotoxicity of taxifolin in HaCaT cells. The results are presented as mean ± S.D. of control density... 19
Fig. 2. Taxifolin suppressed mRNA expression of pro-inflammatory cytokines on TNF-α induced HaCaT cells. (A) Cytotoxicity of taxifolin... 21
Fig. 3. Taxifolin regulated chemokines such as CXCL8 and CCL20 on TNF-α induced HaCaT cells. (A) CXCL8 were increased by TNF-α and... 23
Fig. 4. Activation of IκB by TNF-α was inhibited by taxifolin. (A) Phosphorylation of IκB was induced by TNF-a but inhibited by taxifolin,... 25
Fig. 5. Taxifolin significantly inhibited phosphorylation of MAPK proteins such as p38 and ERK in TNF-α-activated HaCaT cells. (A) The... 27
Fig. 6. Taxifolin inhibited the activation of STAT3 in TNF-α stimulated HaCaT. (A) The expression of p-STAT3 was confirmed by western blot.... 29
Fig. 7. Taxifolin suppressed mRNA expression of cytokines on TNF-α/IL-17A/IFN-γ induced HaCaT cells. (A) Cytotoxicity of taxifolin was... 31
Fig. 8. Taxifolin suppressed mRNA expression of CXCL8 on TNF-α/IL-17A/IFN-γ indicated HaCaT cells. (A) CXCL8 and (B) CCL20 were... 33
Fig. 9. Taxifolin down-regulated the phosphorylation of IκB on TNF-α/IL-17A/IFN-γ indicated HaCaT cells. (A) phospho-IκB protein levels... 35
Fig. 10. Taxifolin was unable to suppress the activation of MAPK such as p38 and ERK in TNF-α/IL-17A/IFN-γ co-stimulated HaCaT cells.... 37
Fig. 11. Taxifolin down-regulated the phosphorylation of STAT3 on TNF-α/IL-17A/IFN-γ indicated HaCaT cells. (A) Phosphorylation of... 39