Title Page
Contents
ABBREVIATIONS 9
국문요약 11
I. INTRODUCTION 13
II. MATERIALS AND METHODS 18
1. Schisandra chinensis seed extracts 18
2. Cell culture 18
3. Cell viability test 18
4. cDNA synthesis and RT-PCR 19
5. Western blot analysis 21
6. Annexin V and PI staining 24
7. Nitric oxidase (Griess Reagent assay) 24
8. Statistical analysis 25
III. RESULT 26
1. Inhibition effects of SCSE on MCF-7 cells growth 26
2. SCSE induced apoptosis in MCF-7 cells 28
3. Effects of SCSE on apoptosis in MCF-7 cells 30
4. Effect of the inhibition of caspase pathway expression after SCSE treatment 36
5. Measurement of nitric oxides 40
6. Effects of oil extracted from SCSE on MAPK pathway-related proteins 44
IV. DISCUSSION 47
V. REFERENCE 53
Abstract 60
Table 1. List of primer sequences and PCR conditions for RT-PCR. 20
Table 2. Antibodies used in the present study. 23
Figure 1. Effect of SCSE on cell viability of MCF-7 cells. 27
Figure 2. Flow cytometry analysis of apoptosis in MCF-7 cells treated with SCSE. 29
Figure 3. SCSE downregulated the anti-apoptotic molecules in MCF-7 cell. 31
Figure 4. SCSE downregulated the anti-apoptotic molecules in MCF-7 cell. 32
Figure 5. Roles of Bcl-2 family in SCSE-induced apoptosis. 33
Figure 6. Role of Bcl-2 family activation in SCSE-induced apoptosis. 34
Figure 7. Schematic diagram illustrating the mechanism of SCSE-induced apoptosis in MCF-7 cells. 35
Figure 8. Schematic diagram illustrating the mechanism of SCSE-induced apoptosis in MCF-7 cells. 37
Figure 9. Role of Caspase activation in SCSE-induced apoptosis 38
Figure 10. Role of Caspase activation in SCSE-induced apoptosis. 39
Figure 11. Schematic diagram illustrating the mechanism of SCSE-induced apoptosis in MCF-7 cells. 41
Figure 12. Nitric oxide production in SCSE-treated MCF-7. 42
Figure 13. Western Blot of Inducible iNOS synthase protein. 43
Figure 14. Schematic diagram illustrating the mechanism of SCSE-induced apoptosis in MCF-7 cells. 45
Figure 15. Roles of MAPK subfamilies in SCSE-induced apoptosis. 46
Figure 16. Schematic diagram illustrating the mechanism of SCSE-induced apoptosis in MCF-7 cells. 52