Title Page
Contents
국문초록 7
ABSTRACT 8
INTRODUCTION 9
MATERIALS AND METHODS 11
1. Chemicals and reagents 11
2. Preparation of 70 % Ethanol Extract of a Euphorbia hirta leaves 12
3. Cell culture and sample treatment 13
4. Cytokine assays 13
5. Quantitative real-time PCR analysis (qRT-PCR) 13
6. Western blot analysis 14
7. Statistical analysis 14
RESULTS 15
DISCUSSION 31
CONCLUSION 34
REFERENCES 35
Figure 1. Effect of ELE on cell viability in HaCaT keratinocytes 16
Figure 2. Effect of ELE on (A) production and (B) mRNA level of TNF-α in TNF-α/IFN-γ-stimulated HaCaT keratinocytes 18
Figure 3. Effect of ELE on (A) production and (B) mRNA level of IL-6 in TNF-α/IFN-γ-stimulated HaCaT keratinocytes 20
Figure 4. Effect of ELE on mRNA level of chemokines, MDC and RANTES, in TNF-α/IFN-γ-stimulated HaCaT keratinocytes 22
Figure 5. Effect of ELE on periostinexpression in TNF-α/IFN-γ-stimulated HaCaTkeratinocytes 24
Figure 6. Effect of ELE on TNF-α/IFN-γ-stimulated JNK phosphorylation in HaCaT keratinocytes 26
Figure 7. Effect of ELE on TNF-α/IFN-γ-stimulated STAT1 activation in HaCaT keratinocytes 29