Title Page
ABSTRACT
Contents
I. INTRODUCTION 15
II. MATERIALS & METHODS 18
1. Preparation of extract 18
2. Cell culture 18
3. Cell viability assay 19
4. Measurement of Melanin Content 20
5. Sandwich Enzyme-Linked ImmunoSorbent Assay 20
6. Real-time PCR 21
7. Western blot 22
8. Measurement of 2,2-diphenylpicrylhydrazyl (DPPH) radical scavenging ability 23
9. Statistical analysis 23
III. RESULTS 24
1. Melanin synthesis and proliferation in B16 cells by sorbuscommixta extract 24
1-1. Proliferation efficacy of sorbuscommixta extract in B16 cells 24
1-2. The increased melanin contents by sorbuscommixta extract in B16 cells 26
1-3. S.T_E extract efficacy of melanin production in B16 cells 30
2. Sorbuscommixta extract efficacy of anti-inflammation 32
2-1. The inhibitory effect of sorbuscommixta extract on IL-6, IL-8, TNF-α mRNA expression in TNFα-stimulated NHDF and HaCaT cells 32
2-2. The inhibitory effect of S.T_DW and S.T_E extract on IL-6, IL-8 and TNF-α mRNA expression in TNFα-stimulated NHDF and HaCaT cells 36
2-3. The inhibitory effect of S.T_E extract on IL-6 and IL-8 protein expression in TNFα-stimulated NHDF and HaCaT cells 39
2-4. Molecular mechanism underlying the pharmacological efficacy of S.T_E extract in TNF-α- stimulated NHDF cells 44
3. Antioxidant potential of sorbuscommixta extract 47
3-1. DPPH assay Efficacy of sorbuscommixta extract 47
IV. DISCUSSION 49
V. CONCLUSION 53
REFERENCES 54
국문 요지 59
Table 1. Inhibition rate of IL-6 and IL-8 expression in TNF-α stimulated... 41
Table 2. Inhibition rate of IL-6 and IL-8 in TNF-α stimulated HaCaT cells... 43
Figure 1. The proliferative efficacy of sorbuscommmixta extract in B16 cells 25
Figure 2. The increased melanin contents by sorbuscommixta extract in... 29
Figure 3. The photo data of the increased melanin production by S.T_Eextract 31
Figure 4. The inhibitory effect of sorbuscommixta extract on IL-6, IL-8... 34
Figure 5. The inhibitory effect of sorbuscommixta extract on IL-6, IL-8... 35
Figure 6. The inhibitory effect of S.T_DW and S.T_E extract on IL-6, IL-8... 37
Figure 7. The inhibitory effect of S.T_E extract on IL-6, IL-8 and TNF-α... 38
Figure 8. S.T_E extract inhibits the production of IL-6 and IL-8 in NHDF cells 40
Figure 9. S.T_E extract inhibits the production of IL-6 and IL-8 in HaCaT cells 42
Figure 10. S.T_E extract inhibited ERK signaling pathway 45
Figure 11. S.T_E extract have no effect of on the IκBα signaling pathway 46
Figure 12. The DPPH radical scavenging ability by S.T_E extract 48