Title Page
Contents
ABSTRACT IN KOREAN 12
I. Introduction 15
II. Materials 19
III. Methods 22
1. Preparation of HA -ATV-L 22
1.1. Preparation of liposome encapsulating ATV (ATV-L) 22
1.2. Conjugation of hyaluronan to ATV-L (HA-ATV-L) 23
2. Characterization of liposome 25
2.1. Size distribution and zeta potential analysis 25
2.2. Encapsulation efficiency of ATV into liposome 26
2.3. Determination of the concentration of conjugated HA to ATV-L 27
3. Cell culture 28
3.1. Culture of MCF-7 adherent cells 28
3.2. Culture of BCSCs (MCF-7 nonadherent cell) 29
3.3. Flow cytometric analysis of MCF-7 adherent cells and BCSCs 30
4. Inhibition of proliferation in MCF-7 adherent cells and BCSCs 31
5. Statistical Analysis 32
IV. Results 33
1. Characterization of liposome 33
1.1. Size distribution and zeta potential analysis 33
1.2. Encapsulation efficiency of ATV into liposomes 37
1.3. Conjugation of hyaluronan to ATV-L 39
2. Cell culture of MCF-7 adherent cells and BCSCs 40
3. Inhibition of proliferation in MCF-7 adherent cells and BCSCs 43
V. Discussion 48
VI. References 52
ABSTRACT 59
Table I. Size distribution and zeta potential analysis 36
Table II. Encapsulation efficiency of ATV into liposome 38
Table III. The IC50 value of cell viability (%) of ATV, ATV-L and...[이미지참조] 46
Table IV. The IC50 value of cell viability (%) of ATV, ATV-L and...[이미지참조] 47
Figure 1. Structures of atorvastatin, various phospholipids and cholesterol. 21
Figure 2. Size distribution of (A) ATV-L and (B) HA-ATV-L. 34
Figure 3. Zeta potential of (A) ATV-L and (B) HA-ATV-L 35
Figure 4. Images of (A) MCF-7 adherent cells and (B) BCSCs... 41
Figure 5. Characterization of phenotypes for (A) MCF-7 adherent... 42
Figure 6. Inhibition of proliferation of ATV formulations on MCF-7... 44
Figure 7. Inhibition of proliferation of ATV formulations on BCSCs... 45
Scheme 1. Preparation of HA-ATV-L 24