Title Page
Contents
ABSTRACT 8
INTRODUCTION 10
MATERIALS AND METHODS 13
1. Cell culture 13
2. THP-1 differentiation 14
3. Quantitative reverse transcription polymerase chain reaction (RT-qPCR) 14
4. Western blot 16
5. Migration assay 17
6. Invasion assay 17
7. Cell proliferation assay 18
RESULTS 19
1. Macrophage-conditioned media increased the migration and invasion of ASCs. 19
2. IL-1β and TGFβ1 were the most increased inflammatory cytokines in PMA-differentiated THP-1 macrophage. 21
3. IL-1β and TGFβ1 had marginal effect on migration and invasion of ASCs. 22
4. Macrophage-conditioned media increased re-adhesion of ASCs, but decreased proliferation of re-adhered ASCs. 23
DISCUSSION 38
REFERENCES 41
ABSTRACT IN KOREAN 44
Figure 1. Migration and invasion of ASCs were increased by THP-... 27
Figure 2. mRNA expression levels of inflammatory cytokines in... 30
Figure 3. Treatment of IL-1β or/and TGFβ1 had marginal impact on... 32
Figure 4. Macrophage-conditioned media increased re-adhesion of... 36
Figure 5. Macrophage-derived humoral factors attract ASCs to a... 37