Title Page
Contents
ABSTRACT 10
INTRODUCTION 12
MATERIALS AND METHODS 15
1. Breast cancer patient tissues 15
2. Total RNA-sequencing and analysis 15
3. Dataset analyses 16
4. Cell culture 16
5. Transfection 17
6. RNA extraction and qRT-PCR 17
7. Cell viability assay 18
8. BrdU incorporation assay 18
9. Colony formation assay 19
10. Transwell cell migration and invasion assay 19
11. Nuclear and Cytoplasmic extraction 19
12. Western blotting 20
13. Statistical analysis 20
RESULTS 22
1. AC138028.6 is highly upregulated in TNBC. 22
2. AC138028.6 facilitates cell proliferation, colony formation, migration, and invasion. 27
3. AC138028.6 affects KLF4 expression and mTOR signaling pathway. 30
4. Overexpression of KLF4 decreases cell proliferation, colony formation, migration, and invasion. 35
5. AC138028.6 exerts oncogenic function by suppressing KLF4 expression. 38
DISCUSSION 42
REFERENCES 45
ABSTRACT IN KOREAN 48
Table 1. Information of qRT-PCR primer 21
Figure 1. Identification of metastasis, recurrence related lncRNAs in TNBC 26
Figure 2. AC138028.6 promotes cell proliferation, colony formation,... 29
Figure 3. KLF4 is a downstream target of AC138028.6... 34
Figure 4. Increased KLF4 expression mitigates cell proliferation, colony... 37
Figure 5. Downregulation of KLF4 is essential for oncogenic role of AC138028.6 41