Title Page
Contents
ABSTRACT 8
I. INTRODUCTION 10
II. MATERIALS AND METHODS 13
1. Cell culture 13
2. Reagents and antibodies 13
3. Plasmids and RNA interference 14
4. Reverse transcription quantitative polymerase chain reaction (RT-qPCR) 15
5. Immunoblot 16
6. Proliferation and wound healing assay 17
7. Invasion assay 18
8. Immunoprecipitation 18
9. Immunofluorescence and live cell imaging 19
10. Statistical analysis 20
III. RESULTS 21
1. Ly6K is elevated in human cervical cancer tissue and cells 21
2. Loss of Ly6K inhibited cancer cell proliferation, migration, and invasion 26
3. Loss of Ly6K decreases TGF-β-induced Smad signaling 32
4. Loss of Ly6K inhibits TβRI degradation 37
5. Loss of Ly6K decreases colocalization of TβRI and endocytic proteins, CAV-1 and clathrin 41
IV. DISCUSSION 48
V. REFERENCES 51
VI. ABSTRACT IN KOREAN 55
Figure 1. Steps of gene selection in this study 23
Figure 2. Expression of Ly6K in various human clinical cancers 25
Figure 3. Ly6K is associated with cell proliferation, migration, and invasion 31
Figure 4. Ly6K binds to TβRI, and regulate the signaling pathway 36
Figure 5. Ly6K is involved in endocytic pathway 40
Figure 6. Ly6K stimulate TGF-β receptor endocytosis through... 47