Title Page
Contents
ABSTRACT 8
I. INTRODUCTION 11
II. MATERIALS AND METHODS 16
1. Cell culture 16
2. Transfection 16
3. RNA and Protein preparation 17
4. qRT-PCR 17
5. Western Blot 18
6. Immunocytometry 18
7. Co-Immunoprecipitation (Co-IP) 19
8. Antibodies 20
III. RESULTS 21
1. TAZ is involved in DNA damage response signaling. 21
2. Influence of DDR stability to primary cilium length. 27
3. Recovery of primary cilium length induced by TAZ defection. 31
4. MCM2 is upstream of TAZ. 35
IV. DISCUSSION 41
V. REFERENCES 45
VI. ABSTRACT IN KOREAN 49
Figure 1. Decreased γH2AX, DDR initial signal, in TAZ knock-down... 24
Figure 2. Decreased ATM, DDR mediator kinase, in TAZ knock-down... 26
Figure 3. Unchanged pattern of shorter primary cilium length by TAZ... 30
Figure 4. Rescued length of primary cilia by double knock-down of TAZ... 34
Figure 5. MCM2 regulates TAZ in post-transcriptional and indirect manner 37
Figure 6. MCM2 is involved in DDR signaling in similar pattern to TAZ 40