Title Page
Contents
1. Introduction 5
2. Methods 9
2.1. Cell culture 9
2.2. Transfection 9
2.3. Target labeling and hybridization to microarray 10
2.4. Data acquisition and analysis 11
2.5. Quantitative real-time PCR (qRT-PCR) 11
2.6. Invasion and migration assays 12
2.7. Luciferase assay 13
2.8. The Cancer Genome Atlas (TCGA) program 13
2.9. Statistical analysis 14
3. Results 15
3.1. Expression levels of LINC00240 and EGFR were suppressed by miR-7-5p 15
3.2. miR-7-5p suppressed cell invasion and migration in NSCLC 16
3.3. Expression of miR-7-5p was regulated by LINC00240 16
3.4. LINC00240 as an miR-7-5p sponge regulator affected cell invasion and migration 17
3.5. LINC00240 was up-regulated in lung squamous carcinoma 18
4. Discussion 19
5. Conclusions 25
References 33
국문초록 40
ABSTRACT 42
Table 1. Top 20 of IncRNAs differentially expressed in miR-7-5p overexpressed H1299. 26
Figure 1. Expression level of miR-7-5p and LINC00240 in NSCLC cell line, and miR-7-5p suppresses expression level of LINC00240, EGFR, cell invasion... 27
Figure 2. miR-7-5p suppresses cell invasion and migration in NSCLC. (A-D) Expression level of miR-7-5p, LINC00240 and EGFR in Hcc1438 with... 28
Figure 3. LINC00240 regulates miR-7-5p and EGFR in NSCLC. (A-D) Expression level of LINC00240, miR-7-5p and EGFR in LINC00240... 29
Figure 4. LINC00240 regulates cell invasion and migration in NSCLC. (A-D) Expression level of LINC00240, miR-7-5p and EGFR in LINC00240... 30
Figure 5. LINC00240 directly interact miR-7-5p. (A) The sequence alignments of miR-7-5p and binding site with miR-7-5p within the LINC00240. (B-C)... 31
Figure 6. TCGA database analysis of LINC00240 (A) and EGFR (B) expression difference between tumor and normal tissues. **P < 0.01 and... 32