Title Page
Contents
List of Abbreviations 5
I. Introductions 8
Alzheimer's disease. 8
Microglia. 9
Selenium-Binding Protein 1. 10
Purpose statement. 11
II. Methods & Materials 12
Animals 12
Genotyping of 5xFAD transgenic mice 12
Immunoblotting 14
Preparation of frozen section of mouse brain 16
Thioflavin-S staining 16
Immunofluorescence and image acquisition 16
Image analysis 18
Statistical analysis 19
III. Results 22
Upregulation of SELENBP1 in the brains of Alzheimer's disease model mice. 22
Upregulation of SELENBP1 in microglial cells of the dorsal subiculum after 3 months. 29
Correlation between SELENBP1 expression and morphological quantitative parameters of microglia in the dorsal subiculum. 34
Correlation between SELENBP1 expression and five morphological quantitative parameters of microglia in the dentate gyrus at 6 months. 44
IV. Discussions 48
V. Conclusions 52
VI. Reference 53
Abstract 59
Figure 1. Genotyping for selecting of 5xFAD TG mice. 13
Figure 2. Validation of SELENBP1 antibody in vitro. 15
Figure 3. Strategy for fractal analysis. 21
Figure 4. Identification of Aβ plaques in the brains of 5XFAD TG... 23
Figure 5. Expression levels of SELENBP1 were not changed in the dorsal subiculum of... 24
Figure 6. SELENBP1 upregulation in the dorsal subiculum of 5XFAD TG mice at 3 months. 25
Figure 7. SELENBP1 upregulation in the dorsal subiculum of 5XFAD TG mice at 6 months. 26
Figure 8. Expression levels of SELENBP1 were not changed between TG and WT mice at 3... 27
Figure 9. SELENBP1 upregulation in the dentate gyrus of 5xFAD TG mice at 6 months. 28
Figure 10. Increased numbers of microglia and astrocytes in the dorsal... 30
Figure 11. SELENBP1 upregulation was observed in the IBA-positive microglia in the... 31
Figure 12. Number of SELENBP1-upregulated astrocytes was lower than SELENBP1 -non-... 32
Figure 13. SELENBP1 upregulation was observed in the IBA-positive microglia in the... 33
Figure 14. SELENBP1 upregulation was mainly observed in reactive... 37
Figure 15. The morphological changes of microglia in the dorsal... 38
Figure 16. The morphological changes of microglia in the dorsal... 39
Figure 17. Correlation of the IBA-1 intensity with morphological... 40
Figure 18. Correlation of SELENBP1 intensity with morphological... 41
Figure 19. The morphological changes of microglia in the dorsal... 42
Figure 20. Correlation of SELENBP1 intensity with morphological... 43
Figure 21. SELENBP1 signal was observed in the all IBA1-positive... 45
Figure 22. The morphological changes of microglia in the dentate... 46
Figure 23. Correlation of SELENBP1 intensity with morphological... 47