표제지
목차
I. 서론 8
1.1. 항산화 8
1.2. 레티놀 10
1.3. 난용성 물질의 가용화기술 13
가. 고분자 마이셀 14
나. 리포좀 15
다. 자가미세유화시스템 16
1.4. 가용화기술을 이용한 레티놀 소재 개발 및 연구목적 18
II. 재료 및 실험방법 20
2.1. 재료 및 시약 20
2.2. 효력시험 21
가. DPPH free radical scavenging activities. 21
나. In vitro tyrosinase inhibition assay 22
2.3. 오일 검색 및 용해도 측정 23
2.4. 적합성 실험 24
2.5. 레티놀의 고분자 마이셀 제조 32
2.6. 미세유화측정실험 34
2.7. 입도측정 35
2.8. 안정성 평가 36
2.9. HPLC 분석조건 37
2.10. Cryo-Scanning electron microscope(Cryo-SEM) 측정 38
2.11. 피부투과 39
III. 실험결과 40
3.1. 효력시험 40
가. DPPH free radical scavenging activities. 40
나. In vitro tyrosinase inhibition assay 44
3.2. 용해도 측정 및 적합성 실험 48
3.3. 미세유화측정실험 50
3.4. 입도측정 54
3.5. 안정성 평가 56
3.6. Cryo-Scanning electron microscope(Cryo-SEM) 측정 61
3.7. 피부투과 63
III. 결론 68
V. 참고문헌 69
Abstract 76
Table 1. Pharmacological properties of Retinol 12
Table 2. Compositions of micelle of oils and various surfactant-cosurfactant mixtures. 25
Table 3. Compositions of micelle of oils and various surfactant-cosurfactant mixtures. 26
Table 4. Compositions of micelle of oils and various surfactant-cosurfactant mixtures. 27
Table 5. Compositions of micelle of oils and various surfactant-cosurfactant mixtures. 28
Table 6. Compositions of micelle of oils and various surfactant-cosurfactant mixtures. 29
Table 7. Compositions of micelle of oils and various surfactant-cosurfactant mixtures. 30
Table 8. Conpositions of phase state. 31
Table 9. Finally selected formulation through various tests. 33
Table 10. DPPH radical scavenging power of ascorbic acid and retinol. 41
Table 11. Value of 50% inhibitory concentrations of Ascorbic... 42
Table 12. Niacinamide, Arbutin, Retinol melanin synthesis inhibitory ability. 45
Table 13. Value of 50% inhibitory concentrations of Niacin... 46
Table 14. Solubilization capacity of retinol with various oils. 49
Table 15. Average particle size of retinol analyzed by... 55
Table 16. The concentration of absorbed retinol was measured... 65
Figure 1. Vitamin A Type Chemical Structure. 11
Figure 2. Solubilization technology. 17
Figure 3. Development of a delivery system to improve the... 19
Figure 4. The process of encapsulating poorly soluble drugs using... 32
Figure 5. DPPH radical scavenging activities of retinol and... 43
Figure 6. Tyrosinase inhibition activities of Retinol and Ascorbic acid. 47
Figure 7. Staining test using methylene blue: Mixture... 53
Figure 8. Stability test of retinol at 25℃ for 24 weeks of micelle... 58
Figure 9. Stability test of retinol at 40℃ for 12 weeks of micelle... 60
Figure 10. Cryo-SEM(Quorum Technologies PP3000T)Image of… 62
Figure 11. The concentration of retinol absorbed was made by... 66
Figure 12. The content of retinol remaining on the membrane... 67