This study was performed to investigate the anti-inflammatory effect of Vaccinium oldhamii branches extract in lipopolysaccharides (LPS)-induced RAW 264.7 cells. In order to confirm the antiinflammatory effect, the nitric oxide (NO) production, which is a representative indicator of inflammmation, was measured, and then the expression of proteins and mRNA of inflammatory enzymes inducible nitric Oxide synthase (iNOS) and cyclooxygenase 2 (COX-2) were measured. As a result, hexane and ethyl acetate fractions showed the highest anti-inflammatory activity by inhibiting NO production of respectively 69.0% and 48.4% at 50 ㎍/mL. In addition, The expression of iNOS protein and mRNA were significantly inhibited. COX-2 significantly inhibited mRNA expression, but there was no significant difference in protein expression. Furthermore, The secretion of interleukin 6 (IL-6), which is an inflammatory cytokine observed as high concentrations in rheumatoid arthritis (RA), were significantly inhibited. And mRNA expression was also inhibited. The inhibitory effect of Janus kinase (JAK), a major signaling pathway of cytokines, was measured by biochemical assay. The inhibitory effect was confirmed only in ethyl acetate fractions. These results indicate that hexane and ethyl acetate fractions of Vaccinium oldhamii branches have anti-inflammatory effect. Especially ethyl acetate fractions may exert anti-inflammatory activity through the inhibiting JAK signaling. Therefore, Vaccinium oldhamii branches extract has potential as a therapeutic agent for inflammatory diseases including rheumatoid arthritis.