Title Page
Contents
Abstract 7
List of Abbreviations 9
Introduction 10
1.1. Application of DNA Markers 12
1.2. Nuclear DNA Polymorphism 12
Materials and Methods 18
2.1. Sample collection and preservation 18
2.2. Genomic DNA extraction 20
2.3. Polymerase chain reaction and sequencing 20
2.4. Full mitochondrial genome isolation 22
Results 24
3.1. Gene organization in the mitochondrial DNA 24
3.2. Protein-coding genes 26
3.3. Ribosomal RNA and transfer RNA genes 27
3.4. Non-coding region 30
3.5. Construction of the Phylogenetic tree for Silurus soldatovi 32
Discussion 34
Conclusion 36
References 38
Table 1. The Gene organization of complete mitochondrial genome of Silurus soldatovi 25
Fig. 1. The Soldatov's catfish, Silurus soldatovi habitat (www.geologypage.com). 17
Fig. 2. Sample collection site (maps.google.com). 19
Fig. 3. A putative secondary structure of twenty-two tRNA genes of Silurus soldatovi... 29
Fig. 4. The Mitochondrial gene map of Silurus soldatovi. 31
Fig. 5. The Phylogenetic relationship of Silurus soldatovi in the family siluridae 33