Title Page
Contents
Abstract 6
Introduction 8
Materials & Methods 10
Preparation of BMP2-conjugated SPs(BCSPs) 10
Fabrication of scaffold and mechanical test 10
Isolation and culturing of adipose-derived stem cells(ADSCs) 10
Alkaline phosphatase(ALP) staining 12
Analysis of mRNA by real-time polymerase chain reaction(qPCR) 13
Protein level measurements by western blot 14
Statistical analysis 15
Results 16
Mechanical test of scaffold 16
Evaluation of BCSPs 20
Measurement of ALP expression levels 21
Effect of BCSPs on cell signaling pathways 24
Discussion 26
References 30
초록 37