표제지
목차
국문초록 5
Abstract 8
I. 서론 15
II. 연구 목적 18
III. 연구 대상 및 방법 20
1. 연골 결손의 동물 실험 모델 20
2. 연골세포의 분리 및 배양 21
3. 이식전 연골세포와 피브린 매트릭스(fibrin matrix)내의 세포분포 관찰 22
4. 연골 결손 부위에 피브린 매트릭스를 이용한 동종연골세포의 이식과 자가 골수 세포 유도 22
5. 결손 부위로부터 재생된 조직의 육안적 관찰 및 생검 23
6. 통계 분석 24
IV. 결과 25
1) 육안 소견 (Gross Appearance of Defect) 25
2) 조직학적 소견 (Histologic and Immunohistochemical Appearance of the Regenerated Cartilage Tissues) 25
V. 고찰 28
VI. 결론 35
VII. 참고문헌 36
Table 1. International Cartilage Repair Society Macroscopic Evaluation of Cartilage Repair 43
Table 2. Modified O'Driscoll Histologic score 44
Figure 1. Photograph of fluoresecnt-dye labeled chondrocytes which are distributed in fibrin based gel. 45
Figure 2. Macroscopic evaluation of the defective areas on articular cartilage from the alloCI, autoBMS, and control groups 3 months after treatment (A, alloCI; B, autoBMS; and C, control). 46
Figure 3. International Cartilage Repair Society (ICRS) evaluation scores of articular cartilage regeneration in alloCI or autoBMS treatment group along with its respective control. 47
Figure 4. Histological analysis of the defect site of the rabbit's knee in untreated group at 3 months after treatment (A, Hematoxylin-Eosin ; B, Masson's trichrome ; C, safranin O; D, Immunohistochemical stain for... 48
Figure 5. H&E staining analysis of the cartilage defect sites from alloCI-treated (A), autoBMS-treated (B), and control (C) groups at 3 months after treatment. 49
Figure 6. Immunohistochemical staining for collagen type II of tissue sections from alloCI-treated (A), autoBMS-treated (B), and control (C) groups at 3 months after treatment. 50
Figure 7. Masson's trichrome staining of tissue sections from alloCI-treated (A), autoBMS-treated (B), and control (C) groups at 3 months after treatment. 51
Figure 8. Safranin-O fast green staining of tissue sections from alloCI-treated (A), autoBMS- treated (B), and control (C) groups at 3 months after treatment. 52
Figure 9. Quantitative histologic evaluation of the regenerated cartilage tissues using modified O'Driscoll grading system scores at 3 months after treatment with alloCI or autoBMS with their respective controls. 53