표제지
국문초록
목차
I. 서론 12
II. 재료 및 방법 14
1. 실험재료 14
1) 산삼복합약침액의 조제 14
2) 3T3-L1 및 Macrophage cell 15
3) 실험 동물 15
2. 실험방법 16
1) 세포실험 16
(1) 세포독성 측정 16
(2) NO 분비 측정 16
(3) 3T3-L1의 분화 억제 측정 17
(4) Macrophage cell의 항염증 효과 측정 18
2) 동물실험 20
(1) 군 분류 및 비만유도 20
(2) 취혈 및 약침처치 22
(3) 체중 변화 기록 23
(4) 조직 적출 및 채혈 23
(5) 간 및 신장 기능 측정 23
(6) 혈중 지질농도 분석 24
(7) 간조직의 지질함량 측정 24
(8) 혈중 인슐린농도 측정 24
(9) 혈중 Leptin함량 측정 24
3) 통계분석 25
III. 연구결과 26
1. 세포실험 26
1) 세포독성 26
2) NO 분비 29
3) 3T3-L1의 분화 억제 32
4) 3T3-L1에서 Adiponectin, PPAR-r 발현 34
5) Macrophage cell의 항염증 효과 36
(1) iNOS발현과 COX-2 36
(2) NF-κB 39
2. 동물 실험 41
1) 체중의 변화 41
2) 간, 신장 무게 및 부고환 주위 지방량 43
3) AST, ALT, r-GTP 및 BUN 농도 45
4) 지질농도 47
5) 혈중 인슐린농도 49
6) 혈중 Leptin농도 51
IV. 고찰 53
V. 결론 64
참고문헌 66
Abstract 78
Table 1. Composition of the experimental diet 21
Table 2. Classification of laboratory animals 22
Table 3. Changes in food intake, body weight gain and food efficiency ratio of the mice fed a high fat diet treated wild ginseng complex pharmacopuncture for 10 weeks. 42
Table 4. Changes in liver, kidney and epididymal adipose tissue weights of mice fed a high fat diet treated wild genseng complex pharmacopuncture for 6 weeks(g/100g body weight) 44
Table 5. Activities of AST, ALT, BUN and glucose contents in body of mice fed a high fat diet containing injection wild ginseng complex pharmacopuncture for 10 weeks. 46
Table 6. Effects of Wild Ginseng Complex Pharmacopuncture in the body lipid and glucose levels in high fat diet-induced obese mice. 48
Fig. 1. Experimental design 22
Fig. 2. Effect of wild ginseng complex pharmacopuncture on cell viability in NIH/3T3 mouse fibroblast. 27
Fig. 3. Effect of wild ginseng complex pharmacopuncture on cell viability in Raw264.7 mouse macrophage. 28
Fig. 4. Effect of wild ginseng complex pharmacopuncture on NO production and inflammatory mediator production in LPS stimulated NIH/3T3 mouse fibroblast cells. 30
Fig. 5. Effect of Wild Ginseng Complex Pharmacopuncture on NO production and inflammatory mediator production in LPS stimulated Raw264.7 mouse macrophage cells. 31
Fig. 6. On Post-MDI 8 days, cultures were fixed and stained for triacylglycerol with Oil Red O (Original magnification ×20). 33
Fig. 7. Wild ginseng complex pharmacopuncture's anti-obesity gene expression analysis in 3T3-L1. 35
Fig. 8. Effects of sample on iNOS expression in LPS-treated Raw 264.7 cells. 37
Fig. 9. Effects of sample on COX-2 expression in LPS-treated Raw 264.7 cells. 38
Fig. 10. Effects of sample on NF-κB expression in LPS-treated Raw 264.7 cells. 40
Fig. 11. Insulin levels in plasma. 50
Fig. 12. Leptin levels in serum. 52