표제지
ABSTRACT
목차
I. 서론 11
II. 연구사 14
1. 가축 육종 및 분자 육종 14
2. 분자 표지를 이용한 선발 육종 기법 17
III. 재료 및 방법 19
1. 공시재료 19
2. Genomic DNA 추출 방법 19
3. 후보유전자 선정 및 SNP 탐색, PCR(유전자 증폭) 20
4. Sequencing(염기서열 분석) 22
5. PCR-RFLP 23
6. 통계적 분석 24
IV. 결과 25
1. 유전자 내 SNP 탐색 25
2. PCR-RFLP에 의한 한우 DECR1 유전자형 분석 및 경제형질과의 연관성 분석 34
3. Haplotype에 대한 경제형질과의 연관성 분석 38
V. 고찰 40
VI. 참고문헌 42
Table 1. DECR1 gene primer information for PCR analysis 21
Table 2. Restricted enzyme digestion for amplified products of each loci 23
Table 3. Distribution of genotypes and alleles for DECR1 gene in this study 27
Table 4. Detail information for amino acid mutation of the Hanwoo DECR1 gene 31
Table 5. Linkage disequilibrium (LD) among polymorphisms in bovine DECR1 gene 32
Table 6. Genotype and Gene Frequencies of G20788A, A23262G by PCR-RFLP with Nla III, HpyCH4 IV 36
Table 7. Least squares means and standard errors for economic traits of G20788A genotype in DECR1 gene 36
Table 8. Least squares means and standard errors for economic traits of A23262G genotype in DECR1 gene 37
Table 9. Haplotype Frequencies and standard errors of G20788A, A23262G in DECR1 gene 38
Table 10. Least squares means and standard errors for economic traits of Haplotype in DECR1 gene 39
Fig 1. SNP genotype of DECR1 region in Hanwoo 25
Fig 2. SNP map of Hanwoo DECR1 gene 30
Fig 3. Linkage Disequilibrium among the DECR1 polymorphisms 33
Fig 4. A 2 % agarose gel displying a HpyCH4 IV restriction digest on an amplified portion of Hanwoo DECR1 exon 5. Lane 1, 100 bp ladder ; lanes 1, 6 are AG; lanes 2, 4 are AA; lanes 3, 5 are GG 34
Fig 5. A 2 % agarose gel displying a Nla III restriction digest on an amplified portion of Hanwoo DECR1 exon 2. Lane 1, 100 bp ladder ; lanes 1 are AA; lanes 2 are GA; lanes 3 are GG 35