Title Page
Contents
I. Introduction 8
II. Purpose 11
III. Patients and Methods 12
Patients and specimens 12
Surveillance 13
Immunohistochemistry 13
Quantitative analysis of TILs 14
Statistical analysis 15
IV. Results 16
Immunohistochemical characteristics 16
Correlation of immunohistochemical variables with clinicopathologic features 17
Prognostic factors 17
V. Discussion 20
VI. References 26
VII. Appendix 31
국문초록 53
Table 1. Descriptive statistics of immunohistochemical variables(n=87). 31
Table 2. Classification of colon cancers according to TIL density (n=87). 32
Table 3. Correlation between TIL and clinicopathologic characteristics. 33
Table 4. Univariate analysis of factors associated with overall and disease-free survival. 34
Table 5. Univariate analysis for the predictors of overall survival anddisease-free survival(results from mean cutoff value). 35
Table 6. Multivariate Cox proportional hazards model for the predictors ofoverall and disease-free survival (results from mean cutoff value). 36
Table 7. Multivariate cox proportional hazards model for prognostic significance of pathologic features and T cell marker density ondisease-free survival. 37
Fig. 1. Representative examples of immunohistochemical findings of intraepithelial and stromal infiltrating lymphocytes. Intraepithelial CD3+ T cells (A), stromal CD3+ T cells (B), intraepithelial CD25+ Tcells (C), stromal CD25+ T cells (D), intraepithelial CD45RO+ T cells...(이미지참조) 38
Fig. 2. Kaplan-Meier analysis of disease-free survival for tumor infiltrating CD45RO+(A,B), CD3+(C,D) and FOXP3+ (E,F) in association with stromal and intraepithelial density.(이미지참조) 40
Fig. 3. Kaplan-Meier analysis of overall survival for tumor infiltrating CD45RO+(A,B), CD3+(C,D) and FOXP3+(E,F) in association with stromal and intraepithelial density.(이미지참조) 44
Fig. 4. Kaplan-Meier survival plots for the 87 colon cancer patients as a function of TIL density. Significant differences in overall survival were found between the low- and high-density groups of CD3+, CD45RO+, CD25+, and FOXP3+ by log-rank tests. Survival differences were...(이미지참조) 48