Title page
Abstract
국문초록
Contents
Introduction 14
Materials and Methods 22
Preparatlion of fetal mouse pancreatic tissue 22
Culture of fetal pancreas 23
Immunocytochemical analysis 24
RT-PCR analysis 25
Measurement of insulin secretion by ELISA 27
Result 28
General histology of fetal pancreas 28
Expression of the transcription factors during development of fetal pancreas 29
Development of fetal pancreatic stem cells during culture 33
Nestin expression in fetal pancreatic stem cells 33
Differentiation of insulin producing cells from fetal ductal cell culture 37
The self-renewal of fetal pancreatic stem cells 38
Development of the insulin-producing cell in the ductal explants 41
Morphological analysis of the dome-like cyst and secondary buds 41
Identification of endocrine cell markers in the fetal pancreatic stem cells 43
Morphometric analysis of insulin-producing cells 47
Secreted insulin and insulin mRNA expression in the culture of fetal pancreas 47
Discussion 51
Reference 59
Fig. 1. Morphological features and expression of transcription factors in pancreas at various stages of fetal development 32
Fig. 2. Isolation of fetal pancreas and profiles of cultured cells at different fetal stages 35
Fig. 3. Nestin expression in the cultured cells of the fetal pancreas 36
Fig. 4. Transformation of the insulin producing cells from the epithelilal explants and their self-replicability 40
Fig. 5. Development of the insulin-producing cell in the ductal explants during the process of culture 44
Fig. 6. Morphological analysis of the dome-like cyst and secondary buds. 45
Fig. 7. Expression of non-beta cell markers in the fetal pancreatic stem cells 46
Fig. 8. Quantification of insulin-producing cell differentiation, insulin mRNA expression and insulin secretion 50