目次
Ⅰ. 緖論 7
Ⅱ. 實驗材料 및 方法 9
1. 材料 9
2. 方法 10
1) 檢液의 調製 10
2) 子宮筋腫組織의 준비 10
3) 子宮筋腫組織으로 부터 細胞培養 및 凍結保存 11
4) 子宮筋腫細胞의 成長抑制 效果 12
5) 子宮筋腫細胞에서의 MAP kinase 活性의 變化 12
6) Western Blotting 14
7) 子宮筋腫細胞에서의 Apoptosis관련 bcl-2 유전자의 발현 15
Ⅲ. 成績 17
1. 子宮筋腫細胞의 成長抑制에 미치는 影響 17
2. 單層培養된 子宮筋腫細胞의 成長抑制에 미치는 影響 20
3. 子宮筋腫細胞에서의 MAP kinase activity 變化 23
4. Phosphorylated MAP kinase 活性變化 26
5. 子宮筋腫細胞에서의 Apoptosis관련 bcl-2 遺傳子의 發現 29
Ⅳ. 考察 32
Ⅴ. 結論 39
參考文獻 41
國文抄錄 47
ABSTRACT 49
表目次
Table 1. Components of Sobokchukeo-tang 9
Table 2. In vitro growth of human uterine myomal cells treated with Sobokchukeo-tang at various concentrations. 18
Table 3. In vitro growth of human uterine myomal monolayered cells treated with different concentrated Sobokchukeo-tang in the media 21
그림目次
Fig. 1. Comparison of growth of human uterine myomal cells treated with different concentrated Sobokchukeo-tang in DMEM medium for 3-day cultivation 19
Fig. 2. Comparison of in vitro growth human uterine myomal cells treated with different concentrated Sobokchukeo-tang in DMEM medium compare to control (10% FBS) medium 22
Fig. 3. MAP kinase activity of cultured human uterine myomal cells treated with different concentrated Sobokchukeo-tang in DMEM media for 3 days 24
Fig. 4. Comparison of MAP kinase activity of human uterine myomal cells cultured different concentrated Sobokchukeo-tang for 3 days 25
Fig. 5. Erk-2 activity, type for phosphorylated MAP kinase, in cultured human uterine myomal cells treated different concentrated Sobokchukeo-tang for 3 days culture 27
Fig. 6. Comparison of Erk-2, phosphorylated type of MAP kinase ,activity of human uterine myomal cells cultured different concentrated Sobokchukeo-tang for 3 days 28
Fig. 7. Bcl-2, appoptosis related gene, expression of cultured human uterine mayomal cells treated with different concentrated Sobokchukeo-tang for 3days 30
Fig. 8. Comparison of bcl-2 activity of human uterine myomal cells cultured different concentrated Sobokchukeo-tang for 3 days 31