目次
Ⅰ. 緖論 6
Ⅱ. 實驗材料 및 方法 8
1. 材料 8
1) 藥材 8
2. 方法 9
1) 檢液의 調製 9
2) 子宮筋腫 組織의 준비 9
3) 子宮筋腫組織으로 부터 細胞培養 및 凍結保存 10
4) 生存細胞數의 算定 11
5) 子宮筋腫細胞의 形態 觀察 12
6) Flow cytometric analysis 12
Ⅲ. 成績 13
1. 濃度에 따른 子宮筋腫細胞의 死滅에 미치는 影響 13
2. 時間에 따른 子宮筋腫細胞의 死滅에 미치는 影響 15
3. 세포의 형태학적 관찰 17
4. 細胞週期의 變化 20
Ⅳ. 考察 23
Ⅴ. 結論 32
參考文獻 33
國文抄錄 39
ABSTRACT 40
表目次
Table Ⅰ. Components of Banchong-san 8
Table Ⅱ. In vitro inhibitory effect of uterine myomal cells treated with Banchong-san at various concentration 14
Table Ⅲ. In vitro inhibitory effect of uterine myomal cells treated with Banchong-san at various time periods. 16
그림目次
Figure 1. Inhibitory effect of Banchong-san on uterine myomal cells at various concentration 14
Figure 2. Inhibitory effect of Banchong-san on uterine myomal cells for various time periods 16
Figure 3. Photomicrograph of uterine myomal cells according to Banchong-san for various time periods 18
Figure 4. Flow cytometry analysis of uterine myomal cells according to FBS percentage for 48hr 21
Figure 5. Flow cytometry analysis of uterine myomal cells according to Banchong-san for various time periods 22