표제지
감사의 글
CONTENTS
ABSTRACT 8
INTRODUCTION 10
MATERIALS AND METHODS 20
Parasites and DNA Extractions 20
AP-PCR and Cloning of Individual Worm-specific Products 20
Preparation of DNA Probes 21
Southern Blot Hybridization 22
Dot-blot Analysis 22
DNA-DNA Hybridization 23
Construction and Screening of Genomic DNA Library 23
Screening of cDNA Library by PCR Methods 24
Locus-Specific Typing 24
Inter-Retrotransposon Amplified Polymorphism(IRAP) 25
Sequence Analysis 25
Phylogenetic Analysis 26
RESULTS 28
I. Isolation and Characterization of a Novel Retrotransposon 28
Isolation of RAPDs by AP-PCR 28
Intragenomic Distribution of CsRn1 Copies 32
Identification of CsRn1 Master Copies 40
The Mobile Activity of CsRn1 Master copy 49
The Evolutionary Relationship of CsRn1 with Other Retrotransposons 52
II. The Distribution Pattern of CsRn1 among C. sinensis individual Genomes 57
Phylogenetic Analysis of CsRn1 LTR Sequences from Multiple Loci 57
Subfamilies of CsRn1 element 62
Comparison of Sequence Divergence between LTRs and rt Regions within CsRn1 Subfamilies 67
Locu-specific Typing of CsRn1 Intergrations among C. sinensis Individual Worms 70
Sequences in the CsRn1-containing Loci 71
The Target Sites for Integration of CsRn1 Elements 74
III. Comparative Studies on CsRn1 elements in the Hepatic Trematodes 76
Homology Level of Total Genomic DNAs between C. sinensis and O. viverrini 76
Distributions of CsRn1 in the Genomes of C. sinensis and O. viverrini 77
IRAP analysis among individuals of C. sinensis and O. viverrini 80
Sequence Variations within Several Regions of CsRn1 Elements 82
Phylogenetic Relationship among CsRn1 Elements 87
DISCUSSION 93
REFERENCE 102
영문 논문 제출서 113
영문 인준서 114
초록 115